冰茶栓对星形胶质细胞增殖、凋亡及细胞周期的影响  

Effects of Bingcha Embolus on the proliferation,apoptosis and cell cycle of astrocytes

作　　者：严继贵[1] 童晔玲[2] 何国浓[2] 俞丽霞[2] 王泽时[2] 

机构地区：[1]安徽医学高等专科学院 [2]浙江中医药大学,浙江杭州310053

出　　处：《中成药》2007年第11期1594-1597,共4页Chinese Traditional Patent Medicine

基　　金：国家九五中医药科技攻关项目;编号:96-906-10-01(02)

摘　　要：目的:应用血清药理学观察冰茶栓(BCS)对体外培养星形胶质细胞(Astrocyte AST)增殖、凋亡及细胞周期的影响。方法:原代培养SD乳鼠大脑皮层AST,将纯化后的细胞经免疫细胞化学鉴定后,随机分为药物组(高、中、低剂量)和对照组,加入相应血清共培养48h后,MTT比色法观察AST的增殖情况,流式细胞仪检测AST的凋亡率和细胞周期的变化。结果:MTT结果显示,冰茶栓高、中剂量组较空白血清组细胞数量明显减少(P<0.01,P<0.05),低剂量组与空白血清组比较无显著差异;流式细胞仪检测结果显示,冰茶栓组细胞凋亡比例较对照组明显增加(P<0.01);冰茶栓组G1期细胞比例较对照组明显提高(P<0.01),S期和G2/M期细胞比例较对照组明显降低(P<0.01)。结论:冰茶栓对体外培养的星形胶质细胞增殖具有明显的抑制作用,并可诱导其凋亡,抑制其由G1期向S期和G2/M期的转化。AIM： To study the effects of Bingcha Embolus on the proliferation, apoptosis and cell cycle of as- trocytes in vitro in terms of serum pharmacology. METHODS ： The cerebral cortex astrocytes of neonatal Sprague- Dawley rats were cultivated in vitro, and the purified astrocytes were randomly derided into Bingcha Embolusgroups （ high dose, middle dose, low dose） and control group after being identified by means of immunocytochemi- cal method. After the incubation of 48 h with sera, MTF was employed as a proper method to detect the prolifera- tion of astrocytes. The ratio of apoptosis and different cell cycle phases were measured by Flow Cytometry. RE- SULTS： The results of MTF showed that the cell number of Bingcha Embolus groups （high dose, middle dose） were obviously fewer than that of the control group （ P 〈 0.01, P 〈 0.05 ）, but there was no much difference be- tween the low dose group and control group; the results of Flow Cytometry showed that the ratio of apoptosis in Bingcha Embolus groups was higher than that of control group （P 〈 0.01 ）, the ratio of cells on G1 phase was high- er in Bingcha Embolus groups than that in the control group （P 〈0.01 ）, and the ratios of cells on S phase and G2/ M phase were lower in Bingcha Embolus groups than that in the control group （ P 〈 0.01 ）. CONCLUSION ： Bingcha Embolus obviously inhibits the proliferation of astrocytes incubated in vitro, induces their apoptosis, and prevents the cell transformation from G1 phase to S phase and G2/M phase.

关 键 词：冰茶栓 血清药理学 星形胶质细胞 流式细胞仪 大鼠 

分 类 号：R285.6[医药卫生—中药学]