RP-HPLC同时测定银杏达莫注射液中4种成分的含量  

Determination of the four components in Ginkgo-Leaf-Extract Dipyridamole Injection by HPLC

作　　者：毕雨萌[1] 孙国祥[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2007年第11期1620-1623,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立银杏达莫注射液中双嘧达莫、槲皮素、山柰素和异鼠李素4种成分的反相高效液相色谱分析方法。方法：以SCIENHOME KromasilC18柱（250mm×4．6mm，5μm），甲醇-0．4％磷酸溶液（55：45，v／v）为流动相，柱温（32±0．1）℃，于360nm测定银杏达莫注射液中上述4种成分的含量。结果：双嘧达莫、槲皮素、山柰素和异鼠李素分别在21．7～261．0μg/mL，8．0～96．0μg/mL、7．75～93．0μg/mL及5．75～69．0μg/mL内峰面积与浓度呈良好的线性关系。精密度（RSD分别为1．16％，1．35％，1．21％和1．58％）良好。4种成分的平均回收率分别为100．3％，100．O％，99．9％，99．8％。结论：本实验建立了可同时测定双嘧达莫、槲皮素、山萘素和异鼠李素4种成分的RP-HPLC方法。该方法简便、准确，可用于银杏达莫注射液的质量控制。AIM： To simutaneously determinate the four components of dipyridamole, quercetin, kaempferol and isorhamnetin in Ginkgo-Leaf-Extract Dipyridamole Injection by the reversed-phase high performance liquid chromatographic （RP-HPLC）. METHODS： Ginkgo-Leaf-Extract Dipyridamole Injection was determined by RP- HPLC on Scienhome Kromasil C1s （250 mm × 4.6 mm, 5 μm） column. The mobile phase consisted of methanol and 0.4% phosphoric acid solution （55： 45, v/v）, the flow rate of 0.80 mL/min and UV detection wavelength at 360 nm were set to determine the contents of the four components. RESULTS： There were good linear relation- ships between peak area and concentration of the four components, and the calibration curves were linear in the ranges of 21.7 - 261.0 μg/mL for dipyridamole, 8.0 - 96.0 μg/mL for quercetin, 7.75 - 93.0 μg/mL for kaempferol, 5.75 -69.0 μg/mL for isorhamnetin. The precisions for dipyridamole, quercetin, kaempferol and isorhamnetin were 1.16%, 1.35%, 1.21% and 1.58%, respectively. The average recoveries were 100.3%, 100.0%, 99.86% and 99.80%, respectively. CONCLUSION ： The four components of the dipyridamole, quer- cetin, kaempferol and isorhamnetin in Ginkgo-Left-Extract Dipyridamole Injection are determined at the same time, and the method is simple, sensitive and accurate.

关 键 词：双嘧达莫 槲皮素 山柰素 异鼠李素 银杏达莫注射液 RP-HPLC 

分 类 号：R927.2[医药卫生—药学]