肾康丸对体外大鼠系膜细胞增殖、细胞外基质分泌和细胞周期的影响  

Effect of Shenkang Pills on Proliferation,Extracellular Matrix Secretion and Cell Cycle of In-vitro Cultured Rat Mesangial Cells

作　　者：肖炜[1] 马云[2] 魏连波[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]南方医科大学南方医院药学部,广州510515

出　　处：《广州中医药大学学报》2007年第6期494-498,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：珠江医院院长基金(编号:200501)

摘　　要：【目的】观察肾康丸对体外高糖培养的大鼠系膜细胞(MC)增殖、细胞外基质分泌(ECM)和细胞周期的影响。【方法】将16只Wistar雄性大鼠随机分为4组,即正常血清组,开博通组(剂量为5 mg/kg),肾康丸高、低剂量组(剂量分别为2.4、1.2 g/kg),灌服相应药物7 d,分离制备药物血清。将体外高糖(30 mmol/LGlu)培养的MC分为5组:高糖模型组(30 mmol/LGlu),正常血清组(体积分数5%正常血清),开博通组(体积分数5%开博通含药血清)及肾康丸高、低剂量组(体积分数5%肾康丸高、低剂量含药血清),另设低糖模型组(10 mmol/LGlu)。培养72 h后,采用四甲基偶氮唑盐(MTT)法测定各组细胞增殖,双抗夹心酶联免疫吸附(ELISA)法检测上清纤维连接蛋白(FN)含量,流式细胞仪检测细胞周期。【结果】高糖培养的MC显著性增殖,上清FN含量显著性增加,并出现细胞周期异常,肾康丸能抑制MC增殖,减少FN分泌,并使G0/G1期细胞比例显著性增加,而S期及G2/M期细胞比例减少。【结论】肾康丸可通过调整MC细胞周期异常,进而抑制高糖诱导的MC增殖和ECM分泌。[ Objective ] To observe the effect of Shenkang Pills （SP） on proliferation, extracellular matrix （ECM） secretion and cell cycle of in-vitro cultured rat mesangial cells （ MC ）. [ Methods ] Sixteen Wistar male rats were randomized into 4 groups： normal serum group, Capoten （5 mg/kg） group, high- and low-dose SP （2. 4 and 1.2 g/ kg respectively） groups. Except the normal serum group, the rats in other groups received corresponding drugs according to the experimental design, and serum containing drug was isolated and prepared after treatment for 7 days. MC cultured with high glucose （30 mmol/L） in vitro were divided into 5 groups： A （cultured with high glucose 30 mmol/L）, B （cultured with 5% normal serum）, C （cultured with 5% serum containing Capoten）, D and E （cultured with 5% serum containing high-and low-dose SP respectively）. Another model group F （cultured with low glucose 10 retool/L） was also established. After culturing for 72 hours, methyl thiazolyl tetrazolium （MTT） assay was used for the detection of MC proliferation, enzyme-linked immunosorbent assay （ELISA） for fibronectin （FN） content in the culture supernatant and flow cytometer for cell cycle. [ Results ] The proliferation of MC and FN content in supernatant were increased, and abnormal cell cycle was found in high-glucose group A. SP inhibited MC proliferation,increased the percentage of MC at G0/Gl phase, and decreased the percentage of MC at S phase and G2/M phase. [ Conclusion] SP has an inhibition on MC proliferation and ECM secretion induced by high glucose, and its mechanism is probably related with the regulation of abnormal MC cell cycle.

关 键 词：肾康丸/药理学 糖尿病肾病/中药疗法 细胞周期 细胞培养 大鼠 

分 类 号：R285.5[医药卫生—中药学]