SMO保存液对犬肾低温保存期间Na^+-K^+ ATPase的影响  

Effect on Na^+-K^+ ATPase in canine kidneys during cold storage with SMO preservation solution

作　　者：韩秋成[1] 郭闻渊[1] 周智华[1] 朱有华[1] 

机构地区：[1]第二军医大学长征医院解放军器官移植研究所,上海200003

出　　处：《陕西医学杂志》2007年第11期1463-1465,共3页Shaanxi Medical Journal

基　　金：上海市科委基金项目(024919006)

摘　　要：目的:研究SMO保存液对犬肾低温保存期间Na+-K+ATPase的影响。方法:建立犬离体肾脏单纯低温保存模型,对照组用0~4℃HTK保存液和UW保存液对供肾进行灌注和保存,试验组用0~4℃自制SMO保存液对供肾进行灌注和保存,分别于24、48、72h后取皮质标本,进行组织病理学观察及检测Na+-K+ATPase的活性。结果:SMO液组所见组织病理学改变,48h和72h比HTK液组轻,在各时间点与UW液组基本相似。Na+-K+ATPase活性,保存各时点,SMO液组与UW液组无明显差异;保存24h和48h,SMO液组与HTK液组无明显差异;保存72h,SMO液组Na+-K+ATPase活性明显高于HTK液组,存在明显差异。结论:SMO保存液在减轻细胞形态学改变和减缓Na+-K+ATPase活性下降方面显著优于HTK保存液,与UW保存液相当。Objective： To study the effect on Na^＋-K^＋ ATPase in canine kidneys during cold storage with SMO preservation solution. Methods： The model of simple cold storage of isolated canine kidney was set up. Kidneys were flushed and storaged with HTK and UW preservation solution （0～4℃） in the control groups and with SMO preservation solution （0～4℃） in the experimental group, Kidney tissue was sampled at 24,48,72 hrs during cold storage. Histopathology was observed and the activity of Na^＋-K^＋ ATPase was assayed. Results： The histopathological changes of SMO group were similar to UW group at each time point and less obvious than HTK group at 48h and 72h . At each time point, there was no significant difference in the activity of Na^＋-K^＋- ATPase between SMO group and UW group. The activity of Na^＋-K^＋ ATPase in SMO group was higher than that of HTK group and there was significant difference at 72h. Conclusions：SMO was superior to HTK and similar to UW solution with respect to extenuating cell morphology changes and slowing down the degression of the activity of Na^＋-K^＋- ATPase.

关 键 词：肾/生理学 保存 生物学 Na(+)K(+)交换ATP酶/分析 狗 

分 类 号：R285.5[医药卫生—中药学]