灵芝煎剂对亚稀褶黑菇急性中毒大鼠肾脏损伤保护作用的研究  

Protective efficacy of Lingzhi decoction on acute renal injury caused by russula subnigricans in rats

作　　者：肖宫[1] 熊军[1] 罗崇岱[2] 杨可达[3] 张平[4] 肖桂林[3] 

机构地区：[1]中南大学基础医学院2004级临床系,湖南长沙410008 [2]中南大学2005级口腔系 [3]中南大学湘雅医院 [4]湖南师范大学生命科学院毒蕈研究所,湖南长沙410081

出　　处：《湖南中医药大学学报》2007年第5期40-43,共4页Journal of Hunan University of Chinese Medicine

基　　金：中南大学米塔尔基金资助项目(71056)

摘　　要：目的探讨灵芝煎剂对亚稀褶黑菇急性中毒大鼠肾脏的保护作用。方法用SD大鼠20只,随机分为4组,分别为:空白组,采用生理盐水灌胃对照;模型组Ⅰ,采用亚稀褶黑菇毒素灌胃造模;模型组Ⅱ,采用亚稀褶黑菇毒素灌胃造模后,用灵芝煎剂灌胃治疗;灵芝组,采用灵芝煎剂灌胃对照。观察大鼠的存活情况、活动情况、尿颜色、尿量、尿红细胞、尿白细胞、尿蛋白、血液生化(尿素氮、肌酐)、NAG酶以及肾脏病理检查等指标。结果实验所观测的指标包括实验动物的存活情况、活动情况、尿颜色、尿量、尿红细胞、尿白细胞、尿蛋白、血液生化(尿素氮、肌酐)、NAG酶等,模型组Ⅱ与模型组Ⅰ各项指标分别比较,有非常显著差异(P<0.01);病理形态学检查结果提示,模型组Ⅱ的肾脏组织结构损害较模型组Ⅰ明显减轻。结论灵芝煎剂对亚稀褶黑菇中毒大鼠肾脏有明显的保护作用。Objective To investigate the protective efficacy of Lingzhi decoction on acute kidney injury caused by russula subnigricans in rats. Methods 20 female SD rats with russula subnigricans were ramdomly divided into 4 groups as follows, normal group, fed with normal saline, model Ⅰ group, with russula subnigricans, model Ⅱ group, with Lingzhi decoction after modeled by russula subnigricans, and fourth group, with Lingzhi decoction only for comparison. Results All the indexes of living condition, behavior, color and volume of urine, RBC, WBC, protein, blood biochemistry （UN, Cr） and β-N-acetyglocosamidase [NAG] in model Ⅱ group were very different compared with model Ⅰ group （P〈0.01） ; the damages of the cells and tissues of kidney in model Ⅱ group were apparently much lighter than that in model Ⅰ group with pathological examination. Conclusions The Lingzhi decoction is very effective to protect rat＇s kidney damaged by russula subnigricans.

关 键 词：灵芝煎剂 亚稀褶黑菇 SD大鼠 急性中毒 肾脏保护 

分 类 号：R285.5[医药卫生—中药学]