人脐带血间充质干细胞在缺氧缺血性脑病新生鼠脑内的定植  

Human Umbilical Cord Blood Mesenchymal Stem Cells Colonizing in Hypoxic-Ischemic Encephalopathy Rats Brain

作　　者：肖升平[1] 马廉[1] 罗敏洁[1] 蒋学武[1] 黄天华[2] 张艳萍[1] 谢庆东[2] 

机构地区：[1]汕头大学医学院第二附属医院儿科,广东汕头515041 [2]汕头大学医学院生殖医学研究中心,广东汕头515041

出　　处：《实用儿科临床杂志》2007年第21期1655-1657,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省社会发展计划项目资助(2003C34007)

摘　　要：目的探讨脐带血间充质干细胞（MSCs）培养及其在缺氧缺血性脑病（HIE）新生鼠脑内的定植。方法用出生7 d的新生SD大鼠制作HIE模型,同时在当天分别用2种方法（定向注射和尾静脉注射方式）接受经Hoechst 33258标记24 h的MSCs移植,15~30 d观察MSCs存活情况。结果用改良Rice法可制备7日龄新生鼠单侧脑损伤为主的HIE模型;经定向注射和尾静脉注射移植的MSCs能在HIE脑内定植,分布在患侧大脑皮层、海马等部位,并主要以额叶为多,和宿主脑组织融合在一起。结论在体外培养条件下,人脐血MSCs可以生长,当MSCs移植到HIE模型鼠后,细胞能和脑实质融合在一起,并更多地向脑损伤部位迁移聚集。Objective To explore human umbilical cord blood mesenchymal stem cells（MSCs） colonization in hypoxic - ischemic enceph- alopathy（HIE） rats brain. Methods Models of 7 - day - old newborn rats with HIE brain injury were established. Meanwhile, on the same day,MSCs were transplanted with Hoechst 33258 for 24 hours into rats models marked by flurescent nucleofide dye injected through caudal vein or with stereotactic instrument. After 15 -30 days, then MSCs were detected with fluorescene microscope. Results With the improved rice methods, HIE animal model was successfully attained. Majority of MSCs were distributed in the cortex, hippocampus of the lesioned hemisphere, especially in the forehead. And abtained a good fusion with HIE rats brain tissue. Conclusion Human umbilical cord blood MSCs can be cultured, when transplanted into the HIE rats model, they can move into intracranial, and integer with rats brain tissue.

关 键 词：脐带血 干细胞 缺氧缺血性脑病 定植 

分 类 号：R722.1[医药卫生—儿科]