参附注射液对缺氧缺血性脑损伤新生大鼠海马神经元凋亡影响  

Effect of Shenfu Injection on Neuron Apoptosis Caused by Hypoxic-Ischemic Brain Damage in Neonatal Rats′ Hippocampus

作　　者：王军[1] 杨丽娟[1] 何艳芳[1] 陈群娥[1] 张绍美[1] 

机构地区：[1]徐州医学院附属医院新生儿科,江苏徐州221002

出　　处：《实用儿科临床杂志》2007年第21期1658-1659,1677,共3页Journal of Applied Clinical Pediatrics

基　　金：江苏省社会发展科技项目资助(BS2003008)

摘　　要：目的研究参附注射液对缺氧缺血性脑损伤(HIBD)新生大鼠海马神经元凋亡的影响。方法实验分成两部分:1.流式细胞术检测缺氧缺血(HI)前2 h、HI后2、12、24 h,3、7、14和28 d各时间点新生大鼠海马CA1区神经元凋亡率;2.免疫组织化学测HI前2 h、HI后2、12、24 h,3、7、14和28 d各时间点新生大鼠海马CA1区神经元Bcl-2、Bax蛋白表达。建立新生大鼠HIBD模型。每组实验随机分为4组:假手术组(S),9 g/L盐水对照组(C),参附预处理组(P),参附治疗组(SF)。结果SF和P组海马神经元凋亡情况明显低于C组(P<0.01,<0.05),且Bcl-2表达显著增多(P<0.05,<0.01),而Bax表达明显下调(P<0.05,<0.01)。结论参附注射液可明显上调新生大鼠HIBD后海马神经元Bcl-2蛋白表达,下调Bax蛋白表达,减少HIBD新生大鼠海马神经元凋亡发生。Objective To study the effect of shenfu injection on the neuron apoptosis in hippocampal CA1 region of newborn with hypoxic - ischemic brain damage（HIBD）. Methods The experiment included 2 parts. One was to measure the apoptosis rate by the flow cytometry,and the other was to investigate the expression of Bcl - 2 and Bax of neurons in left hippocampal CA1 region. Models of postnatal 7 - day Sprague - Dawley （ SD ） rats with HIBD were established, and were equally divided into 4 groups： sham operation （ group S）, control group （ group C ）, shenfu injection pretreatment（ group P） ,and shenfu injection treatment（ group SF）. The neuron apoptosis rate in hippocampal CA1 region in every group was measured at 2 h before and 2,12,24 h ,3,7,14 and 28 d after hypoxic ischemic（HI） insult. The expressions of Bcl - 2 and Bax were performed by immunohisochemistry. Results The apoptesis of neuron in hippocampal CA1 region in group P and group SF after HI insult was significantly less than that of group C （ P 〈 0.01, 〈 0.05 ）. The expression of Bcl - 2 was markedly higher than that in group C （ P 〈 0.05, 〈 0. 01 ） and the expression of Bax was markedly down - regulated （ P 〈 0.05, 〈 0.01 ）. Conclusion Shenfu injection effectively prevents apoptosis of hippocampus neurons from HI insult by up - regulation of the anti - apoptotic gene Bcl - 2 and down - regulation of the pro - apoptotic gene Bax.

关 键 词：参附注射液 缺氧缺血性脑病 Bcl-2 BAX 凋亡 海马 

分 类 号：R285.5[医药卫生—中药学]