荧光定量聚合酶链式反应法检测婴儿尿液人巨细胞病毒DNA对人巨细胞病毒感染的诊断意义  

Diagnostic Significance of Quantitative Detection of Human Cytomegalovirus DNA in Urine by Fluorescent Quantitative Polymerase Chain Reaction

作　　者：马艳萍[1] 齐莹[1] 吉耀华[1] 刘庆[1] 王继东[1] 孙峥嵘[1] 何蓉[1] 阮强[1] 

机构地区：[1]中国医科大学附属盛京医院病毒研究室,沈阳110004

出　　处：《实用儿科临床杂志》2007年第22期1695-1696,1712,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨荧光定量聚合酶链式反应(FQ-PCR)方法检测婴儿尿液人巨细胞病毒(HCMV)DNA水平对婴儿HCMV感染的诊断价值。方法采用FQ-PCR检测348例临床疑似HCMV感染婴儿尿液中HCMVDNA水平。同时采用化学发光免疫分析法(CLIA)检测婴儿血清HCMV-IgM抗体。在尿液HCMVDNA检测阳性患儿中比较血清HCMV-IgM抗体检测阳性与阴性患儿尿液中HCMVDNA拷贝数差异。结果FQ-PCR检测尿液HCMVDNA的阳性率为41.74%,CLIA检测婴儿血清HCMV-IgM抗体的阳性率为19.83%。2种方法对HCMV感染诊断的符合率为76.7%。前者诊断阳性率显著高于后者(χ2=69.44P<0.01)。在尿液HCMVDNA检测阳性患儿中,IgM抗体阳性组尿液HCMVDNA拷贝数显著高于阴性组(P<0.01)。结论FQ-PCR检测尿液HCMVDNA是早期诊断婴儿HCMV感染的敏感有效的方法。HCMV感染婴儿尿液中高HCMVDNA拷贝数与活动性HCMV感染密切相关;FQ-PCR方法检测尿液HCMVDNA水平对于判断是否为HCMV活动性感染具有一定意义。Objective To evaluate the diagnostic usage of fluorescent quantitative polymerase chain reaction（ FQ - PCR） assay in human cytomegalovirus（HCMV） infection by detecting quantitatively HCMV DNA in urine of infants. Methods Urine and blood samples were collected from 348 infants with suspected HCMV infection. Urine samples were used for detecting HCMV DNA by FQ - PCR. Blood samples were used simultaneously for detecting HCMV IgM antibody by chemiluminescent immunoassay （CLIA） assay. Among the FQ - PCR positive infants, quantitative levels of the HCMV DNA were determined and compared with the infants of HCMV IgM negative group. Results The positive rate of FQ - PCR was 41.7% ,and that of CLIA was 19.8% ,the consistency rate of the two methods for diagnosis of HCMV infection was 76.7%. FQ - PCR assay was superior to CLIA（χ^2 = 69.44 P 〈 0.01 ）. Quantitative levels of HCMV DNA in urine of HCMV IgM positive infants were significantly higher than those of HCMV IgM negative infants（P 〈 0.01 ）. Conclusions High levels of HCMV DNA in urine were closely con＇elated with active HCMV infection. FQ - PCR is a sensitive assay for diagnosis of HCMV infection, and can be used as a diagnostic measure for active HCMV infection.

关 键 词：荧光定量聚合酶链式反应 化学发光免疫分析法 巨细胞病毒 人 

分 类 号：R725.1[医药卫生—儿科]