麻疯树新基因JcPIP的分子克隆与功能研究  

Cloning and Function of a New Aquaporin Gene(JcPIP) from Jatropha Curcas

作　　者：张颖[1] 江璐玎[1] 王云霄[1] 牛蓓[1] 陈放[1] 

机构地区：[1]生物资源与生态环境教育部重点实验室(四川大学生命科学学院)

出　　处：《林业科技开发》2007年第6期12-16,共5页China Forestry Science and Technology

基　　金：国家自然科学基金面上项目(编号:30670204);国际合作项目(编号:2003DFB00005)

摘　　要：从大戟科耐旱植物麻疯树cDNA中克隆得到了一个麻疯树质膜内膜蛋白(PIP)新基因(JcPIP)。其cRNA在非洲爪蟾卵母细胞(Xenopus oocytes)中异源表达后发现细胞膨胀率扩大了10倍。聚类分析表明,麻疯树PIP蛋白与蓖麻、葡萄和菠菜PIP蛋白在进化上有最近的亲缘关系。半定量RT-PCR研究表明该基因在植株的整个生长发育期都有表达。但干旱胁迫下JcPIP基因表达量没有显著变化。Amplified by PCR with degenerated primers, a new plasma membrane intrinsic protein gene （JcPIP） was cloned from the cDNA of Jatropha curcas. The cRNA of JcPIP was hetero expression in Xenopus oocytes as a water channel and increased swelling rate 10 times higher. Phylogenic analysis indicated that PIPs of Jatropha curcas was relation to Ricinus com- munis, Vitis vinifera and Spinacia oleracea closely. Semi-quantitative PCR analysis showed it was expressed in each phase of Jatropha curcas growth. Furthermore, there were no obviously changes in PIPs expression levels under drought stress.

关 键 词：水通道蛋白 功能分析 干旱胁迫 

分 类 号：Q785[生物学—分子生物学]