异丙酚对严重烧伤后大鼠心肌细胞c-fos基因表达及血清肌钙蛋白I浓度的影响  

Effects of propofol on blood serum concentration of myocardial CTn-I and expression of c-fos gene in rat after severe burn

作　　者：吴悦维[1] 杨宗城[2] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037 [2]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《重庆医学》2007年第4期333-334,337,共3页Chongqing medicine

摘　　要：目的研究异丙酚对严重烧伤后大鼠心肌c-fos基因表达及血清肌钙蛋白I浓度的影响,从分子水平探讨严重烧伤后大鼠心肌损伤的保护机制。方法96只大鼠随机分为3组:(1)对照组(严重烧伤后未治疗);(2)严重烧伤后大鼠生理盐水治疗组;(3)大鼠严重烧伤后异丙酚间断治疗组;(4)大鼠严重烧伤后异丙酚持续治疗组,持续靶控静注异丙酚。分别于伤后30、60、360、720min等时间点,测定心肌细胞c-fos基因改变以及肌钙蛋白I的变化。结果异丙酚能显著抑制严重烧伤后大鼠心肌细胞c-fos基因的表达;同时,肌钙蛋白I较对照组亦有显著降低,差异有统计学意义(P<0.01)。结论异丙酚可能对严重烧伤后大鼠心肌具有分子水平保护作用。Objective To study the effects of propofol on rat myocardial CTn-I and expression of c-fos gene in rat after severe burning injury. Methods Ninety-six rats were randomly divided into control group, （NS infusion） and propofol groups （propofol was infused after severe burning,interval-infusion and lasting-infusion）. Total cellular RNA was isolated at 30,60,360,720min af- ter burn. Optical density（OD）value and myocardial CTn-I was measured. Results OD values decreased more in propofol lasting-infusion group than that in control group and interval-infusion group, and myocardial CTn-I decreased significently（P〈0.01）. Conclusion Propofol may significantly decrease the leveal of blood serum concentration of myocardial CTn-I and inhibit the expression of c-fos gene. In the molecular modulation, propofol may protect rat myocardium after severe burning injury.

关 键 词：心肌 C-FOS基因 肌钙蛋白 异丙酚 烧伤大鼠 

分 类 号：R644[医药卫生—外科学]