检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:王毅超[1] 邹全明[1] 任建敏[1] 郭刚[1] 解庆华[1] 刘健[2]
机构地区:[1]第三军医大学临床微生物及免疫学教研室暨重庆市生物制药工程技术研究中心,重庆400038 [2]北华大学附属医院,吉林132011
出 处:《重庆医学》2007年第13期1285-1287,共3页Chongqing medicine
基 金:国家"十五"课题资助项目(2001AA215161);国家"863"课题资助项目(2003AA215020)
摘 要:目的改变壳聚糖-海藻酸钠包裹幽门螺杆菌全菌蛋白微球制备中的各项参数,最终确认微球制备工艺。方法根据形成乳液的溶解性、流动性及黏稠度,选择微球制备过程中海藻酸钠(AGS)浓度及植物油与海藻酸钠的配比,通过比较形成微球的粒径大小,最终确定微球制备中CaCl2溶液的滴定程序及搅拌速率、搅拌时间等实验参数。结果确定了AGS乳液浓度为2%、植物油与AGS乳液配比为2∶8、CaCl2溶液反滴滴定法等MS制备工艺,并确定了搅拌速率800r/min、药物浓度2mg/ml、制备温度25℃、搅拌时间30min等参数。结论依照上述参数可以制备出符合实验要求的微球疫苗。Objective To obtain the technology of Helicobacter pylori whole cell protein encapsulated by chitosan-alginate through the change of the technology parameter. Methods To choose the concentration of the AGS and the ratio between AGS and vegetable oil on the basis of the dissolvability, flowability and mucosity and to determine the speed and time of the mixture and methods of the titration through studying the size of the microsphere, Results Some parameters were definited. The concentration of alginate sodium (AGS) was 2%, the matching of the oil and AGS was 2 : 8, the procedure was reverse titration, The four factors affecting the MS diameter with isoproject method were analyzed. The definited parameters were as follows:the mix speed of 800rpm, drug concentration of 2mg/ml, environment temperature of 25℃ and mix time of 30min. Conclusion The microsphere may be prepared successfully according to the above-mentioned technologic parameters.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.70