抗HER-2嵌合抗体chA21对SKBR3细胞的增殖抑制及凋亡诱导作用  

Effects of anti-HER-2 chimeric antibody chA21 on proliferation and apoptosis of SKBR3 cells

作　　者：薛花[1] 吴强[1] 胡向阳[1] 凌晓光[1] 杨枫[1] 程联胜[2] 刘兢[2] 

机构地区：[1]安徽医科大学病理学教研室,安徽合肥230032 [2]中国科学技术大学生命科学学院,安徽合肥230027

出　　处：《中国药理学通报》2007年第11期1463-1466,共4页Chinese Pharmacological Bulletin

基　　金：国家高技术研究发展计划(863计划)资助项目(No2004AA215260);安徽省自然科学基金资助课题(No03043701);安徽省教育厅自然科学基金资助课题(No2003kj185);安徽医科大学青年科研基金资助项目(No2003ZR15)

摘　　要：目的探讨抗HER-2嵌合抗体chA21在体外抑制高表达HER-2的人乳腺癌SKBR3细胞增殖并诱导其凋亡的作用。方法采用MTT比色法、HE染色、透射电镜、流式细胞术(FCM)、原位末端标记技术(TUNEL)等方法观察和检测chA21对人乳腺癌SKBR3细胞增殖的抑制和凋亡的诱导。结果chA21(0.2～5.4mg.L^(-1))可抑制SKBR3细胞的增殖并诱导其凋亡,其作用呈剂量和时间依赖性。结论chA21在体外可抑制SKBR3细胞的增殖,诱导其凋亡是其主要作用途径之一。Aim To explore the effects of anti-HER-2 chimeric antibody chA 21 on proliferation and apoptosis of SKBR3 cells.Methods MTT colorometric assay,HE staining,transmission electron microscopy,flow cytometry,and TUNEL were used to study the proliferation inhibition and apoptosis induction of SKBR3 cells by chA 21 in vitro.Results Proliferative inhibition rates and apoptotic index of SKBR3 cells were increased in a dose and time dependent manner after exposure to chA21（0.2～5.4 mg·L^-1）.Conclusion chA 21 could remarkably inhibit proliferation of SKBR3 cells in vitro and apoptosis induction may be one of its main mechanisms.

关 键 词：HER-2 嵌合抗体 CHA21 乳腺癌 SKBR3 增殖 凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]