尖吻蝮蛇毒小分子多肽对人卵巢癌A2780细胞增殖和黏附的影响  

Effect of peptide from agkistrodon acutus snake venom on adhesion and proliferation of human ovarian carcimona A2780 cell line

作　　者：雷丹青[1] 李映新[1] 周先果[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西南宁530021

出　　处：《中国药理学通报》2007年第11期1514-1518,共5页Chinese Pharmacological Bulletin

基　　金：广西壮族自治区自然科学基金资助项目(No0575062)

摘　　要：目的探讨尖吻蝮蛇毒小分子多肽(K组分)对体外培养的人卵巢癌A2780细胞株的增殖抑制作用及机制。方法MTT法观察K组分对人卵巢癌A2780细胞增殖的影响;采用光镜、电镜观察细胞形态变化;荧光染色法测定和观察K组分对A2780细胞凋亡影响;采用结晶紫染色法测定K组分对A2780与FN黏附作用的影响。结果MTT显示K组分呈剂量与时间依赖性抑制人卵巢癌A2780细胞株的增殖,电镜观察给药组细胞出现明显凋亡,荧光显微镜下可见细胞形态发生改变,K组分对A2780细胞在FN基质上的黏附有一定的抑制作用,并呈浓度依赖关系。结论K组分对体外培养的人卵巢癌A2780细胞株有较明显的抑制作用,该作用与诱导细胞凋亡和抑制细胞和细胞外基质黏附的能力有关。Aim To investigate the inhibitory effect of peptide from Agkistrodon Acutus snake venom（fraction K）on the growth of human ovarian carcimona A2780 cell line and its mechanism.Methods The A2780 cells were treated with fraction K at different concentrations.The proliferation of A2780 cells was assayed with MTT colorimetric method.The histologi-cal and ultras-tructural changes of cell were scored using light and electron microseopy.The apoptosis of A2780 cells induced by fraction K was studied by fluorescent staining.Crystal violet staining and MTT assay were employed to determine the effect of fraction K on A2780 cells that they had been adhered to fibronectin.Result Proliferation of A2780 cells was inhibited significantly by fraction K in a dose-and time-dependant manner.Under electron-microseop and fluorescence microscope,some A2780 cells underwent a typical apoptosis and morphology changes after 24 hours incubation with fraction K.Fraction K could inhibit the adhesion of A2780 cells to fibronectin in a dose-dependent manner.Conclusion fraction K can inhibit proliferation of A2780 cells,which may be related to the mechanism of inducing cellular apoptosis and cellular adhesion.

关 键 词：尖吻蝮蛇毒 卵巢癌 增殖 黏附 凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]