外源性CGRP对家兔胸腹撞击伤后心肌保护作用的实验研究  

Experimental study of the protection effect of extrinsic CGRP on the myocardial of rabbits with thoraco-abdominal Injury

作　　者：孙海华[1] 张晓华[1] 孙士锦[1] 麻晓林[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所全军战创伤中心,重庆400042

出　　处：《重庆医学》2007年第22期2261-2263,共3页Chongqing medicine

摘　　要：目的探讨外源性应用降钙素基因相关肽(CGRP)对胸腹撞击伤后心肌的保护作用及其可能机制。方法成年新西兰兔随机分为3组,A组:对照组;B组:伤后应用常规剂量CGRP组(0.012μg/kg);C组:伤后应用大剂量CGRP组(0.024μg/kg)。BIM-Ⅳ型生物撞击机撞击动物剑突部致伤。测定3组动物伤前及伤后外周血清LDH、CK、ET值,检测心肌组织MDA、SOD值和心肌细胞内(Ca2+)平均荧光强度,并行大体病理解剖及伤情分析。结果实验动物致伤率达100%。3组实验动物伤后LDH、CK、ET值B、C组非常显著低于A组,差异有统计学意义(P<0.01),C组非常显著低于B组,差异有统计学意义(P<0.01);MDA含量B、C组非常显著低于A组,差异有统计学意义(P<0.01),且C组明显低于B组,差异有统计学意义(P<0.05);SOD活性B、C组非常显著高于A组,差异有统计学意义(P<0.01),且C组明显高于B组,差异有统计学意义(P<0.05),同时心肌细胞内(Ca2+)平均荧光强度B、C组显著低于A组,差异有统计学意义(P<0.01),且C组明显低于B组,差异有统计学意义(P<0.05)。结论CGRP可剂量依赖性抑制ET的升高,纠正MDA和SOD的失衡,同时阻止心肌酶的漏出和心肌内的钙离子超载,对心肌具有一定的保护作用。Objective To investigate the myocardial preservation effect and the possible mechanism of extrinsic CGRP （calcitonin-gene-related peptide） after thoraco-abdomen impact injury. Methods Thirty rabbits divided randomly to 3 groups, Group A： simple impact group ; Group B： normal dosage CGRP- impact group （CGRP 0. 012μg/kg） ; Group C： large dosage CGRP-impact group（CGRP 0. 024μg/kg）. Impact injury was established with BIM-Ⅳ biological impact machine at appendix ensiformis . Detected LDH,CK, ET before and after injury respectively, and determined MDA, SOD activity and the intracellular dissociated calcium ion of myocardial tissue, while observing the pathological change and analysis the traumatic condition. Results All the experiment rabbit were injured. LDH,CK,ET were lower in group B and group C than those in group A （P〈0.01）, and group C was lower than group B （P〈0.01）. MDA exponent of group B and C was lower than that of group A （P〈0.01）, and group C was lower than group B （P〈0.05）. SOD activity in group B and C was higher than that of group A （P〈0.01）, and group C was higher than group B （P〈0.05）. The exponent of intracellular dissociated calcium ion in the heart group B and group C was much lower than that of group A （P〈0.01）, and group C lower than group B （P〈0.01）. Conclusion CGRP dose dependent preventing ET from rising, balancing the activity of SOD and exponent of MDA, and preventing the leak-out of CK and overloading of calcium ion in cardiac muscle, to attenuated the injury of cardiac muscle after trauma.

关 键 词：胸腹撞击伤 降钙素基因相关肽 血管内皮素 心肌保护 新西兰兔 

分 类 号：R641[医药卫生—外科学]