TrxS基因的表达及其对大麦种子淀粉酶活性的影响  

Expression of TrxS and its Effect on Amylase Activities in Barley Seeds

作　　者：卫丽[1] 尹钧[1] 黄晓书[1] 孔维威[1] 邓德芝[1] 

机构地区：[1]河南农业大学/国家小麦工程技术研究中心,郑州450002

出　　处：《中国农业科学》2007年第11期2437-2442,共6页Scientia Agricultura Sinica

基　　金：国家转基因植物研究与产业化专项(JY03-B-19);河南省科技成果转化基金项目(0636000005)

摘　　要：【目的】分析TrxS基因对大麦种子发芽及淀粉酶活性的影响,为TrxS基因对大麦种子代谢的调控提供了理论依据。【方法】以转TrxS基因啤酒大麦株系为材料,从分子水平和生理指标进行分析。【结果】实时荧光RT-PCR检测证明TrxS基因在RNA水平上能够表达;转基因大麦籽粒α-淀粉酶和β-淀粉酶活性明显提高,发芽1-5d转基因大麦种子α-淀粉酶活性比对照分别增加5.86%、75.24%、118.51%、71.02%和22.99%,β-淀粉酶活性分别比对照增加19.29%、14.68%、35.47%、23.62%和2.67%;种子的发芽能力提高。【结论】TrxS基因能够促进种子发芽。[Objective] In order to provide a theoretical basis for the regulation of seed metabolism, the paper analyzed the effect of TrxS gene on barley seed amylase activities seeds during germination period. [Method] Transgenic and non-transgenic barley seeds were used to analyze its function from molecular level and physiological characters. [Result] TrxS gene could express in transgenic barley seeds by the detection of real-time PCR. During germinating days 1-5d, the activities of α- amylase were 5.86%, 75.24%, 118.51%, 71.02% and 22.99% higher than that of non-transgenic seeds, respectively. The activities of β-amylase were 19.29%, 14.68%, 35.47%, 23.62% and 2.67% higher than that of non-transgenic seeds, respectively. Germinating power of transgenic seeds was higher than that of non-transgenic seeds.[ Conclusion ]TrxS gene could improve the germination of barley seeds.

关 键 词：TRXS基因 淀粉酶 活性 实时荧光PCR 发芽 

分 类 号：S512.1[农业科学—作物学]