高温胁迫下转甜椒正义甘油-3-磷酸酰基转移酶基因(GPAT)烟草缓解PSⅡ光抑制  

Tobacco Transformed with Sweet Pepper Sense GPAT Alleviates PSⅡ Photoinhibition Under High Temperature Stress

作　　者：颜坤[1] 陈娜[2] 曲妍妍[1] 郭峰[1] 孟庆伟[1] 赵世杰[1] 

机构地区：[1]山东农业大学生命科学学院/作物生物学国家重点实验室,泰安271018 [2]中国科学院植物研究所,北京100093

出　　处：《中国农业科学》2007年第11期2468-2473,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金(30471053)资助

摘　　要：【目的】探讨类囊体膜脂组成与高温光抑制的关系。【方法】以转甜椒正义甘油-3-磷酸酰基转移酶基因(GPAT)的烟草植株为试验材料,测定了类囊体膜脂组成,高温胁迫后的光合参数和叶绿素荧光参数。【结果】转基因烟草植株类囊体膜的单半乳糖甘油二脂(MGDG),双半乳糖甘油二脂(DGDG),硫代异鼠李糖甘油二脂(SQDG)和磷脂酰甘油(PG)的饱和程度均上升,其中MGDG饱和程度增加了16.2%,上升幅度最为显著。随胁迫温度的升高,转基因烟草植株与野生型植株Fv/Fm,ΦPSⅡ和Pn逐渐下降,但转基因植株下降幅度小于野生型,48℃时差异最为显著。【结论】转基因烟草植株类囊体膜脂脂肪酸饱和度增加,提高了PSⅡ热稳定性,缓解了PSⅡ光抑制。[Objective] In order to investigate the relationship between lipid composition in thylakoid membrane and high temperature-induced photoinhibition. [Method]Tobacco plants transformed with sweet pepper sense GPATwere used to analyze the lipids composition of thylakoid membrane, the photosynthetic and chlorophyll fluorescence parameters after high temperature stress. [Result] The results suggested that the saturated extent of MGDC, SQDC, DGDG and PG in thylakoid membrane of transgenic tobacco plants generally increased. However, the saturated extent of MGDG increased obviously by 16.2%.With stress temperature rising, Fv/Fm, φPS Ⅱ and Pn of transgenic and wild type tobacco plants decreased gradually, but those parameters in transgenic plants decreased less than wild type plants. The significant difference occurred at 48℃. [Conclusion] The increase in saturated thylakoid membrane lipids of transgenic plants enhanced the stability of PS Ⅱ and alleviated PS Ⅱ photo-inhibition under high temperature.

关 键 词：甜椒甘油-3-磷酸酰基转移酶基因 烟草 类囊体膜脂 高温胁迫 PSⅡ光抑制 

分 类 号：S641.3[农业科学—蔬菜学]