哈密瓜细菌性果斑病菌快速检测方法的建立  

Establishment of Rapid Detection Method for the Pathogen of Hami Melon Fruit Blotch

作　　者：回文广[1] 赵廷昌[1] Schaad N W 孙福在[1] 王建荣[3] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室 [2]United States Department of Agricultural Research Service Foreign Disease-Weed Science Research Unit MD 21702 USA [3]新疆生产建设兵团农六师科委

出　　处：《中国农业科学》2007年第11期2495-2501,共7页Scientia Agricultura Sinica

基　　金：国家自然基金项目(30370949);农业部"948"项目(2003-Z51);新疆生产建设兵团博士资金课题资助

摘　　要：【目的】哈密瓜细菌性果斑病在中国为新发生的病害,其病原菌为燕麦食酸菌西瓜亚种(Acidovorax avenae subsp.Citrulli),危害瓜果造成品质下降。该病为典型的种传细菌性病害,因此种子检疫成为防治此病害的重要手段。【方法】通过实验,将生物学、免疫学和分子生物学检测技术有机结合,建立了一套针对哈密瓜果斑病的种子带菌检测方法Bio-IMS-Real-timePCR。【结果】经过人工模拟种子带菌检测实验,结果表明该方法可成功地检测出1000粒种子中的1粒带菌种子(带菌量约为1.04×105CFU/种子,经计算,可以检测的最初浓度为2CFU·ml-1ASCM培养液),且信号较强。【结论】该检测方法的建立,大大提高了对哈密瓜细菌性果斑病菌检测的精度,也为生产实践中哈密瓜果斑病的防治提供了重要的技术支持。[Objective] Hami melon bacterial fruit blotch （HBFB） is a new disease in China and the pathogen is Acidovorax avenae subsp, citrulli, which damages the fruits and lowers their qualities. HBFB is a typical seedborne bacterial disease, and the important prevention and cure method is the quarantine of the seeds with bacteria. [Method] In this study, the immuno-detection was combined with PCR by using the superparamagnetic beads to form Bio-IMS-Real-time PCR, which increase the precision of bacteria detection. The integrated method of bacterial detection was formed through combining the ASCM culturing, IMS and real-time PCR. [Result] As a result, one seed with bacteria （average 1.04× 10^5 CFU/seed） in 1 0013 seeds could be detected using this integrated method. The threshold of A. avenae subsp, citrulli detection for Bio-IMS-Real Time PCR was 2 CFU-ml^-1 in ASCM liquid. [ Conclusion ]This method can greatly increase the precision ofA. avenae subsp, citrulli detection, and provide a important technical suooort for the prevention and control of HBFB.

关 键 词：哈密瓜细菌性果斑病 快速 检测 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]