用斑马鱼检测猪链球菌2型的致病力  

Virulence Detection of Streptococcus suis Type 2 in Zebrafish

作　　者：濮俊毅[1] 黄新新[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095

出　　处：《中国农业科学》2007年第11期2655-2658,共4页Scientia Agricultura Sinica

基　　金：国家"973"项目(2006CB504403)

摘　　要：【目的】猪链球菌2型(Streptococcus suis type2,SS2)菌株致病力各异,以斑马鱼为实验动物,建立了较为简便可靠的SS2致病力检测方法。【方法】选用1005尾AB系斑马鱼(Danio rerio)以检测SS2不同分离株的致病力。检测8株基因型均为mrp+ef+、对猪有致病力的SS2菌株。【结果】结果斑马鱼接种菌株12h后呈败血症病变,96h内对斑马鱼的半数致死量(LD50)在5.36×103至5.01×104cfu之间。上述接种菌株的斑马鱼均从体内重新分离到接种菌。同时检测1株基因型为mrp-ef-、对猪无致病力的SS2菌株,斑马鱼接种后96h内不表现任何病变,亦不出现死亡,对斑马鱼的LD50>106cfu。SS2有毒力株和无毒力株对斑马鱼的LD50差异极显著(P<0.01)。【结论】斑马鱼可作为研究猪链球菌2型菌株感染的动物模型。[Objective] Zebrafish （Danio rerio） is a new infection model for studying mammal pathogens. [Method] One thousand and five 80-day-old AB line inbred zebrafish were grouped and infected intraperitoneally with 8 highly virulent strains （mrp^＋ef^＋） for pigs of Streptococcus suis type 2 （SS2）. [Result] The 50% lethal dose （LDso） were in the range of 5.36×10^3cfu to 5.01×10^4 cfu at 96 h. The SS2 strains caused high morbidity and mortality in zebrafish. Acute septicemia were observed in inoculated fish. The bacteria inoculated could be reisolated from the infected fish. One strain avirulent （mrpef） for pigs was also inoculated but not lethal for fish, and its LD50 was above 106 cfu. There was a significant difference in LD50 in the SS2 virulent strains and avirulent one （P〈0.01）. [ Conclusion ] Zebrafish is useful for further research of SS2 infection as a new animal model.

关 键 词：斑马鱼 猪链球菌2型 半数致死量 

分 类 号：S858.28[农业科学—临床兽医学]