一种基于玻璃-PDMS微流控芯片上气动微泵的研制及其在化学发光分析中的应用研究  

Fabrication of pneumatic micropump on glass-PDMS microfluidic chip and its application to chemiluminescence detection

作　　者：张玲[1] 徐光明[1] 崔群[1] 方群[1] 

机构地区：[1]浙江大学化学系微分析系统研究所,浙江杭州310027

出　　处：《浙江大学学报（理学版）》2007年第6期648-652,共5页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学重大基金资助项目(批准号:20299030)

摘　　要：利用AZ4620光胶制备通道横截面为圆弧形的阳模，采用玻璃与PDMS材料的永久性封合技术，研制了一种玻璃-PDMS复合芯片微流控蠕动型气动微泵，实现单一微泵对多路液流的驱动．实验将该微泵系统应用于K3[Fe（CN）6]的化学发光检测，对4×10～mol／LK3[Fe（CN）6]测定的重现性为0．9％（RSD，n=7），线性范围6×10^-6～6×10^-5mol／L，线性相关系数R^2=0．9990，检出限为8×10^-7mol／L．A novel approach for microfabrication of pneumatic micropump on microfluidic chip was reported by glass- PDMS binding technique. The mold of fluid channels with round cross section was acquired using AZ4620 photore- sist. Double fluid channels were controlled synchronously. The performance of the system was tested by chemilumi- nescence detection. With the device K3 [Fe（CN）6] can be detected at a concentration of 8×10^-7 mol/L and with a RSD of 0.9 % （4 × 10^-5 mol/L,n= 7）, and a linear calibration was obtained from 6 × 10^-6 to 6 × 10-5 mol/L . The linear regression coefficient R^2 was 0. 999 0.

关 键 词：微流控芯片 气动微泵 化学发光检测 

分 类 号：O652[理学—分析化学]