加拿大一枝黄花二萜成分的抗肿瘤活性  

Antitumor activity of diterpenoids from Solidago canadensis L

作　　者：刘晓月[1] 朱宏科[1] 吴世华[1] 陈云龙[1] 刘菲燕[1] 吴平[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310058

出　　处：《浙江大学学报（理学版）》2007年第6期661-664,共4页Journal of Zhejiang University（Science Edition）

基　　金：浙江省计划重点项目(863配套;2003AA223071)

摘　　要：通过对4株人癌细胞系，肝癌SMMC7721，红白血病K562、乳腺癌Bcap37和肺腺癌SPC-A1的体外抑制实验发现，加拿大一枝黄花花序的石油醚、乙酸乙酯浸膏具有较强的体外抑制肿瘤细胞生长的能力,二者对上述4株肿瘤细胞的半数抑制浓度（IC50）低于50μg／mL．依据初筛结果，采用MTT法进行活性成分的跟踪检测，从乙酸乙酯浸膏中分离到两个抗肿瘤活性二萜．由NMR确定它们的结构分别是6β-当归酰克拉文酸和6β-巴豆酰克拉文酸．这两个化合物对4株肿瘤细胞株的半数抑制浓度（IC50）在7～12μg／mL．由于两个化合物对人永生化上皮细胞系HaCaT的毒性较小（IC50〉50μg／mL），加拿大一枝黄花可能成为开发抗肿瘤药物的新来源，是一种具有研究价值的草药．Petrol ether, ethyl acetate and methanol extracts of the inflorescence of Solidago canadensis L. were test- ed in vitro for anti-tumor activity against four tumor cell lines, human myelogenous leukaemia K-562, breast cancer cell line Bcap37, lung adenocarcinoma SPC-A1 , and hepatocellular carcinoma SMMC 7721. Results showed that petrol ether and ethyl acetate extracts were 50% toxic to four human tumor cell lines in the range of t5 and 50 μg/mL. Two known diterpenoids （6β-angeloyloxykolavenic acid and 6β-tigloyloxykolavenic acid） were isolated from the ethyl acetate extracts by bioactivity-guided method . The structures of the two diterpenoids were determined by nuclear magnetic resonance. Their IC50 against four human tumor cells were between 7 and 12μg/mL. The little cytotoxici- ty （IC50〉50μg/mL） of two diterpenoids to human epithelial cell line HaCaT makes Solidago canadensis L. a po- tential candidate for anti-tumor medicine.

关 键 词：加拿大一枝黄花 二萜 抗肿瘤 克拉文酸 

分 类 号：R284.1[医药卫生—中药学]