寒胁迫诱导灰木相思无性系差异蛋白的表达  

Differential protein expression induced by cold stress the clones of Acacia implexa

作　　者：何文锦[1] 陈由强[1] 郭晋隆[2] 林思祖[3] 陈如凯[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350007 [2]农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002 [3]福建农林大学林学院,福建福州350002

出　　处：《福建林学院学报》2007年第4期355-359,共5页Journal of Fujian College of Forestry

基　　金：福建省自然科学基金资助项目(B0510009);福建省林业厅科技攻关项目(闽林综[2003]17号);福建省教育厅科学基金资助项目(JB03124)

摘　　要：本实验提取寒胁迫前后的灰木相思组培苗的水溶性蛋白进行双向电泳和质谱鉴定,分析在寒胁迫条件下蛋白质组的变化。利用ImageMaster 5.0软件分析比较,经基质辅助激光解吸电离飞行时间质谱测定差异蛋白点的肽质量指纹图谱,通过Mascot软件查询Swiss-Prot等数据库,鉴定得到4个显著差异蛋白点,R7、R15、R20表现为上调表达,R17表现为下调表达。结果表明:在寒胁迫条件下灰木相思组培苗存在明显的蛋白质表达差异。There are two different treatment modes in plantlet of Acacia implexa： cold streass culture and normal temperature culture. Water-soluble proteins were isolated from cold stress culture and normal temperature culture of plantet of A. implexa respectively, then profiled by two-dimensional polyacrylamide gel electrophoresis and software of lmageMaster 5. 0, identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） . Their Pepfide Mass Finger Prints （PMF） were obtained. Further Swiss-Prot database search identified four matched proteins. Among them, three proteins were up-regulated while one protein was down-regulated. The results showed that there were definite differences of protein expresion differences between the cold stress culture and normal temperature culture of plantet of A. implexa.

关 键 词：寒胁迫 灰木相思 蛋白质组学 双向凝胶电泳 MALDI-TOF质谱 

分 类 号：Q946.1[生物学—植物学]