小熊猫脑源性神经营养因子(BDNF)基因的克隆与序列分析  

MOLECULAR CLONING AND SEQUENCING OF THE BRAIN DERIVED NEUROTROPHIC FACTOR(BDNF) GENE FROM LESSER PANDA

作　　者：林峰[1] 杨玉华[1] 张义正[1] 陈红卫[2] 费立松[2] 宋云芳[2] 何光昕[2] 张安居[2] 

机构地区：[1]四川大学生物工程系分子生物学实验室 [2]成都动物园

出　　处：《四川大学学报（自然科学版）》1997年第3期367-370,共4页Journal of Sichuan University(Natural Science Edition)

基　　金：成都大熊猫繁育研究基金

摘　　要：参照人的ＢＤＮＦ基因序列设计了一对引物，利用聚合酶链式反应（ＰＣＲ），从小熊猫基因组ＤＮＡ中扩增和克隆到ＢＤＮＦ基因．序列分析表明，小熊猫和人的ＢＤＮＦ基因核苷酸序列同源性为９３％，而与大熊猫ＢＤＮＦ基因的核苷酸序列同源性高达９８％．在推导的多肽序列中，除在前导肽区有两个氨基酸的差异外，小熊猫ＢＤＮＦ的成熟区与人和其他已报道的哺乳动物ＢＤＮＦ成熟区的氨基酸序列完全一致。The authors employed the polymerase chain reaction (PCR) to amplify the full coding region of the BDNF gene from lesser panda ( Ailurus fulgens) genomic DNA directly with the primers based on the sequence of human BDNF gene.The amplified fragment was cloned and sequenced.Sequence analysis showed that the coding region of BDNF gene from lesser panda was highly homologous with that of human and giant panda counterpart genes (93% and 98% homology,repectively).It was found that there were only four amino acid residues showing difference in the prosequence region by comparing the deduced amino acid sequence of BDNF gene of lesser panda with that of human.However,the amino acid sequence of mature BDNF from lesser panda is identical to that of all the reported mammalian BDNFs.

关 键 词：小熊猫 脑源性 神经营养因子 序列分析 基因 

分 类 号：Q959.838[生物学—动物学]