Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria  

作　　者：李永峰 徐菁利 杨传平 任南琪 

机构地区：[1]Chemical Engineering School, Shanghai University of Engineering and Sciences [2]Forestry School, Northeast Forestry University [3]Municipal and Environmental Engineering School,Harbin Institute of Technology

出　　处：《Journal of Harbin Institute of Technology(New Series)》2007年第5期691-694,共4页哈尔滨工业大学学报（英文版）

基　　金：Sponsored by Program of Shanghai Education Committee (Grant No07ZZ156);Key Subject Construction of Shanghai Education Committee(Grant NoP1402); the National Natural Science Fund of China(Grant No30470054)

摘　　要：A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.A method based on PCR amplification of the 16S rRNA gene （rDNA）-23S rDNA intergenic spacer regions （ISR） was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogenproducing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.

关 键 词：Biohydrogen production Hydrogen-producing Anaerobes ISR of 16S-23S rDNA Length polymorphisms 

分 类 号：X506[环境科学与工程—环境工程]