槲寄生多糖的提取分离和含量测定  

Extraction and content determination of polysaccharides in Viscum coloratum

作　　者：王俊[1] 朱一凡[1] 

机构地区：[1]江苏科技大学生物与环境工程学院,江苏镇江212018

出　　处：《中国中药杂志》2007年第22期2387-2390,共4页China Journal of Chinese Materia Medica

基　　金：江苏省高校自然科学研究计划项目(03KJD350026)

摘　　要：目的:对国产槲寄生中的有效成分多糖进行提取分离和含量测定。方法:采用热水提取法提取槲寄生多糖,超滤膜分离-离子交换色谱法分离纯化多糖组分;槲寄生多糖经苯酚-硫酸显色后,在490 nm处进行比色测定。结果:槲寄生药材、槲寄生粗多糖CVPS-Ⅲ和槲寄生精制多糖CVPS-Ⅲ-C中的多糖含量分别为4.93%(RSD1.04%,n=3),43.28%(RSD 1.39%,n=3)和69.55%(RSD 1.62%,n=3),平均回收率为96.07%(RSD 2.54%,n=5)。结论:该法简便、快速、准确、灵敏度好。Objective： To establish a method for extraction and content determination of polysaccharide in Viscum coloratura, Method： Polysaccharide was extracted by hot water, separated by ultrafiltration and ion-exchange chromatography. The content deter- mination was performed at wavelength 490 nm with phenol-sulfuric acid as a chtomo-genic agent. Result： The content of polyaccharide in V. coloratura, CVPS-Ⅲ, and CVPS-Ⅲ-C were respectively 4.93% （RSD 1.04%, n =3）, 43.28% （RSD 1.39%, n =3）, 69. 55% （RSD 1.62% , n =3） , and the average recovery was 96. 07% （RSD 2. 54%, n =5）. Conclusion： The method was simple, rapid, and accurate.

关 键 词：槲寄生 多糖 提取分离 含量测定 

分 类 号：R284.1[医药卫生—中药学]