促肾上腺皮质激素释放激素、c-Fos及间隙连接蛋白43与分娩发动的相关性  

Clinical study of the correlation among the levels of human corticotropin-releasing hormone, c-Fos and connexin-43 at onset of labor

作　　者：吴湘[1] 丁依玲[1] 喻玲[1] 

机构地区：[1]中南大学湘雅二医院妇产科

出　　处：《中华妇产科杂志》2007年第11期737-741,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：湖南省科技厅科研基金(06SK3041)

摘　　要：目的探讨促肾上腺皮质激素释放激素(CRH)、c-Fos 和间隙连接蛋白43(Cx43)在足月妊娠子宫平滑肌细胞中的表达变化及其与分娩发动的相关性。方法选择2006年2—9月在中南大学湘雅二医院住院的足月临产产妇30例(临产组),足月未临产产妇30例(未临产组),因宫颈上皮内瘤样病变行子宫切除术患者30例(对照组)。应用放射免疫方法测定3组妇女静脉血中 CRH 的水平;用核酸原位杂交技术和免疫组化链霉菌抗生物素蛋白-过氧化物酶连接(SP)法检测3组妇女子宫平滑肌细胞中 c-Fos、Cx43 mRNA 及其蛋白的表达水平。结果 (1)CRH 水平在临产组产妇为(81.8±11.9)pmol/L,未临产组为(34.5±18.6)pmol/L,对照组为(1.3±0.7)pmol/L,临产组明显高于未临产组及对照组,未临产组明显高于对照组,差异均统计学意义(P<0.05)。(2)c-Fos mRNA及其蛋白的阳性染色主要定位于子宫平滑肌细胞核内,少量位于细胞质内;子宫平滑肌细胞中 c-FosmRNA 及其蛋白表达水平,临产组分别为5.4±1.7及5.4±1.5,未临产组分别为4.1±0.9及3.9±0.9,对照组分别为1.0±0.4及1.2±0.4,临产组明显高于未临产组及对照组,差异有统计学意义(P<0.05);未临产组也明显高于对照组(P<0.05)。(3)Cx43 mRNA 及其蛋白的阳性染色定位于子宫平滑肌细胞质内;子宫平滑肌细胞中 Cx43 mRNA 及其蛋白表达水平,临产组分别为5.5±1.4及5.6±1.3,未临产组分别为4.1±0.6及4.1±0.5,对照组分别为1.5±0.6及1.1±0.6,临产组明显高于未临产组及对照组,差异有统计学意义(P<0.05);未临产组也明显高于对照组(P<0.05)。(4)3组妇女的 CRH 水平与子宫平滑肌细胞中 c-Fos mRNA 及其蛋白的表达水平呈明显正相关(r_1=0.61,r_2=0.50;P<0.05);与子宫平滑肌细胞中 Cx43 mRNA 及其蛋白的表达水平呈明显正相关(r_3=0.52,r_4=0.68;P<0.05);子宫平滑肌细胞中 c-Fos mRNA 及其蛋白的表达水平与 Cx43 mRNA 及其蛋白的表达水平也呈明显正�Objective To investigate the variation of corticotropin-releasing hormone （CRH） in plasma, the mRNA and protein expression of c-Fos and gap junction gene connexin-43 （Cx43） in the myometrium of term pregnancy women, and to study the correlations among CRH, Cx43 and c-Fos at onset of labor. Methods 30 cases in labor （L group）, 30 cases not in labor （NL group） but in term pregnancy, and 30 cases of non-pregnant （ NP group） women undergoing hysterectomy due to cervical intraepithelial neoplasia were recruited into this clinical study. Radioimmunoassay was employed to measure the concentration of CRH in plasma; in situ hybridization assay and streptravidin-biotin peroxidase （SP） immunohistoehemical techniques were respectively used to detect the expression levels of c-Fos mRNA, Cx43 mRNA and the corresponding proteins. Results （ 1 ） The concentration of CRH in L group （ 81.8 ± 11.9 ） pmol/L was significantly higher than that in NL group （ 34. 5 ± 18.6 ） pmol/L （ P 〈 0. 05 ） , which was significantly higher than that in NP group（ 1.3 ± 0. 7 ） pmol/L（ P 〈 0. 05 ）. （ 2 ） Most of the positive staining of c-Fos mRNA and protein in myometrium were located in cell nuclei and a few were in cytoplasm; and the expression level of c-Fos mRNA and protein were 5.4 ± 1.7 and 5.4 ± 1.5 in L group, 4. 1 ± 0. 9 and 3.9 ± 0. 9 in NL group, 1.0 ± 0.4 and 1.2 ± 0. 4 in NP group, respectively. There was a significant difference among different groups （P 〈 0.05 ）. （3）The positive staining of Cx43 mRNA and protein in myometrium was located in the cytoplasm ; and the expression level of Cx43 mRNA and protein was 5.5 ± 1.4 and 5.6 ± 1.3 in L group, 4. 1±0.6 and 4.1±0. 5 in NL group, 1.5±0. 6 and 1.1±0. 6 in NP group, respectively. There was a significant difference among different groups （ P 〈 0.05 ）. （4） In each group, there was a positive correlation between plasma CRH concentration and the expression of c-Fos mRNA and protein in myometrium （r1 = 0

关 键 词：分娩 产科 促肾上腺皮质素释放激素 原癌基因蛋白质c—Fos 连接蛋白43 

分 类 号：R714[医药卫生—妇产科学]