家兔制备传染性法氏囊病抗体的探讨  

Proparation of Antibody to Infectious Bursal Disease with Domestic Rabbis

作　　者：程太平[1] 荣俊[2] 彭本英[1] 

机构地区：[1]长江大学动物科学学院,湖北荆州434025 [2]长江大学生命科学学院

出　　处：《畜牧兽医杂志》2007年第6期20-22,共3页Journal of Animal Science and Veterinary Medicine

摘　　要：采用自制传染性法氏囊病（IBD）灭活油乳剂疫苗免疫家兔，第2次免疫后4～5周，血清IBD抗体水平可达1-64。对每一份血清样品以两种方法进行纯化，即亲和层析与盐析-透析中，IBD抗体丢失量约15％左右。血清和透析所得液体经亲和层析，收集的混合液中IBD抗体水平均为1／8，蛋白含量在1．07％～1．25％，但血清样品比透析液样品的蛋白水平高4．8％～10．7％。对血清样品和透析液样品亲和层析后收集的混合液的蛋白水平进行t检验，t=1．64，t0.05（3）=3．182，t〈t0.05（3），P〉0．05，差异不显著。结果表明，血清样品直接进行亲和层析比盐析-亲和层析节省时间和成本，并可减少血清抗体的丢失量。Domesic rabbits were injected with killed emulison vaccine of infectious bursal disease （IBD）made by oneself. Serum IBD antibody level of domestic rabbits come up to 1/64 after secondary vaccination in 45 weeks. Each serum specimen was purified with affinity chomatography and salting--out and dialysis. The serum specimens and the solution form salting --out and dialysis were purified with affinity chromatography. IBD antibody level is 1/8 and protein level is 1.70%-1.25% in collected mixture solution. The t test was taken between the protein level in collected mixture solution from the serum specimens purified with affnity chromatography and that one from the dialysis solution puriried with affinity chromatography,and t= 1. 64 ,t0.05（3）= 3. 182 ,t〈t0.05（3） ,P〉0.05 ,the difference is not remarkable. It is make known that the purification to serum speci- mens by affinity chromatography is saving at time and cost in comparison with salting--out adding Qffinity chromatograhy and the first purification way may avoid IBD antibody lost.

关 键 词：传染性法氏囊病 血清抗体 亲和色谱 蛋白A--Sepharose--4B 

分 类 号：S853.733[农业科学—临床兽医学]