陆地棉品种抗黄萎病性状的分子标记及其辅助选择效果  

Molecular Markers of Verticillium Wilt Resistance in Upland Cotton(Gossypium hirsutum L.) Cultivar and Their Effects on Assisted Phenotypic Selection

作　　者：王芙蓉[1] 刘任重[1] 王留明[1] 张传云[1] 刘国栋[1] 刘勤红[1] 马小波[1] 张军[1] 

机构地区：[1]国家棉花改良中心山东分中心山东省农业科学院棉花研究中心,济南250100

出　　处：《棉花学报》2007年第6期424-430,共7页Cotton Science

基　　金：国家转基因专项基金项目(JY03-B-05-02);山东省优秀中青年科学家奖励基金项目(03BS133)

摘　　要：利用综合性状优良、适应性广、抗黄萎病的鲁棉研22号与渐渗了海岛棉优异纤维基因的鲁原343组配杂交组合,对F2:3家系在不同发病时期进行黄萎病鉴定,并以SSR标记对抗黄萎病性状进行定位分析。在棉花发病相对较轻的7月份检测到1个位点(qVWR-16-1a)与抗黄萎病有关;在发病较重的8月份,检测到3个QTLs位点与抗黄萎病有关,其中1个QTL(qVWR-16-1b)与7月份检测到的位点qVWR-16-1a为同一位点,能解释的表型变异为10.27%,与NAU751连锁较紧密,另外2个与BNL1395和BNL3590连锁较紧密的位点qVWR-16-2b、qVWR-2-1b,能解释的表型变异分别为10.8%和13.78%。利用检测到的与3个QTL位点连锁较紧密的SSR标记对杂交后代辅助鉴定,发现标记NAU751和BNL1395的抗性基因型均能显著增加后代的黄萎病抗性,两个标记的抗性基因型聚合后,后代抗性水平提高极显著。分子标记辅助抗病选择应用于优异纤维渐渗系杂交后代的鉴定中,可以培育出既优质又抗病的棉花新品种。Genetic population of F2 and its corresponding F2：3 lines derived from a hybridization combination of a Verticillium wilt disease resistant cultivar of pest-resistant transgenic upland cotton （Gossypium hirsutum L. ）, crossed to a Verticillium wilt disease sensible upland cotton line （Luyuan 343） with high fiber quality genomic components introgressed from G. barbadense L. , were used to evaluate the resistance of Verticillium wilt disease and then dissected the resistance genes by SSR markers with the phenotype data of different developmental stages in cotton growth season, qVWR-16-1a, which was detected with the data of the vigorously developmental stage, is located between the two markers, BNL2986 and NAU751. in the fragment of chromosome 16 with 5.73 cM to NAU751,It accounted for 16.53% of the phenotypic variation and the resistante proved to be from the genotype of resistance parent. Meanwhile, with the data of the late stage of the cotton growth season, three QTLs were detected to be related to Verticillium wilt resistance, qVWR-16-1b is located of the in the same interval to qVWR-16-1a with 1.73 cM to marker locus NAU751 ,It accounted for 10.27% of the phenotypic variation.Another locus named qVWR-16-2b is located in another interval between BNL1604 and BNL1395 in the same chromosomal fragment, with 1. 39 cM to BNL1395. It accounted for 10.8% of the phenotype variation. Another QTL named qVWR 2-1b located in the interval between BNL3950 and BNL3971 with only 0.01 cM to BNL 3950 in the fragment of chromosome 2,and accounted for 13.78% of the phenotypic variation. Evaluation on the disease resistance of some offspring （F5） in the breed ing population showed that pyramiding the resistant genotypes of marker NAU751 and BNL1395 can significantly improve the Verticilliurn wilt disease resistance. It was concluded, in this paper, molecular markers can be used to improve Verticillium wilt disease resistance in the process of breeding using the high fiber quality introgression line by assistant selec

关 键 词：陆地棉 抗黄萎病 SSR QTLS 分子标记辅助选择 

分 类 号：S562[农业科学—作物学]