维A酸对银屑病患者外周血T淋巴细胞增殖及JAK3表达的影响  

The Effect of Retinoid Acid on the Proliferation of Peripheral T Lymphocytes and the Expression of JAK3 in Psoriasis

作　　者：李瑾[1] 顾军[1] 毕新岭[1] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433

出　　处：《中国皮肤性病学杂志》2007年第11期641-643,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金资助(30271197)

摘　　要：目的探讨第三代维A酸（芳维A酸氨丁三醇）和第二代维A酸（依曲替酸）对银屑病患者T淋巴细胞增殖的影响及其机制。方法抽取银屑病患者外周血，分离T淋巴细胞，经不同浓度药物处理后，台盼蓝染色检测细胞活力，用改良M1Tr法检测淋巴细胞的增殖能力；分别用Real．timePCR和蛋白印迹方法检测JAK3，STATS的mRNA和蛋白表达水平。结果芳维A酸氨丁三醇与依曲替酸在10^-9-10^-6浓度范围内，均对银屑病患者外周血T淋巴细胞的增殖有抑制作用，且呈剂量依赖性，芳维A酸氨丁三醇比依曲替酸抑制作用更明显；两种药物均可下调银屑病患者T淋巴细胞JAK3基因的表达，且芳维A酸氨丁三醇的下调作用更显著，但STAT5蛋白表达无变化。结论芳维A酸氨丁三醇和依曲替酸可明显抑制银屑病患者外周血T淋巴细胞的增殖，其机制可能与下调JAK3表达有关。Objective To study the effects of third-generation tretinoid acid （ Arotinoid Trometamol） and the second gener- ation tretinoid acid （Acitretin） on the proliferation of T lymphocytes in patients with psoriasis and related mechanisms. Methods T cells were isolated from psoriasis, after treated with different concentrations of drug. Trypan blue staining cells, lymphocyte prolif- eration were detected by modified MTT assay. Then the mRNA and protein expression changes of JAK3 and STATS were separately detected by real-time PCR and Western blot method. Results Arotinoid trometamol and Acitretin both inhibited the proliferation of psoriatic peripheral T lympbocytes in accordance with the drug concentration range of 10^-9 - 10^-6 The effect was dose-dependent, Arotinoid trometamol for inhibition was more obvious than Acitretin;two drugs reduced the expression of JAK3 in the T lymphocytes from psoriasis. Arotinoid trometamol downward effect could be more pronounced, but STATS protein expression did not change. Conclusion Arotinoid trometamol and Aeitretin could inhibit the proliferation of T lympbocytes in psoriasis. It might be related to the down-regulating of JAK3 expression.

关 键 词：T淋巴细胞 维A酸 银屑病 JAK3 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]