食品中40种有机磷和氨基甲酸酯农药多残留快速检测技术研究  

A study on rapid determination of 40 organophosphorus and carbamate pesticide multi-residues in food

作　　者：赵亚华[1] 何学芳[1] 李勇[1] 张金涛[1] 

机构地区：[1]安徽省马鞍山市疾病预防控制中心,安徽马鞍山243000

出　　处：《中国卫生检验杂志》2007年第11期1938-1940,1967,共4页Chinese Journal of Health Laboratory Technology

基　　金：马鞍山市卫生局第六次科技攻关基金资助项目(2005)

摘　　要：目的：建立食品（蔬菜、水果、粮食、茶叶）中40种有机磷和氨基甲酸酯农药多残留快速检测方法。方法：样品用乙腈提取,固相萃取CARB/NH2小柱净化,双柱-NPD气相色谱法同时定性、定量测定40种有机磷和氨基甲酸酯农药残留。结果：40种农药在HP-5柱和DB-17柱上分离效果较好,其线性范围较宽,并在此浓度范围内显良好的线性关系（r=0.99～0.9999）,在蔬菜、水果、粮食、茶叶中3个浓度添加水平平均回收率为61.4%～116.4%,变异系数为1.7%～15.7%,最低检出浓度为0.005～0.1 mg/kg。结论：方法准确,重现性、精密度好,杂质干扰少,且简便快速,成本低,适用范围广。该方法符合农药多残留测定方法要求。Objective： To set up a rapid determination for 40 organophosphorus and earbamate pesticide multi -residues in food. Methods：The sample was extracted with aeetonitrile, then pre -purified by passing through Solid -phase Extraction CARB/ NH2. The residues of 40 organophosphorus and earbamate pesticide in food （vegetables, fruits, foodstuffs and teas） were identified and quantified simultaneously by gas chromatography with dual -column and NPD. Results：In HP- 5 Column and DB -17 Column,40 pesticides were separated from each other,and the liner range of standard curve were broad and the liner relationship of standard curve were good（ r =0. 99 ～ 0. 9999 ）. The average recoveries of the three levels of 40 organophosphorus and earbamate pesticide were 61.4% ～ 116. 4% in vegetables,fruits,foodstuffs and teas. The coefficient of variation （CV） was 1.7% ～ 15.7% and the range of minimum detection limits was 0. 005 ～ 0. 1 mg/kg. Conclusion：This method is of good accuracy, high repeatability,precision and less interferences from impurities. Meanwhile it is simple and fast and the cost is low and apply scope is wide. This method has met the requirement of analysis method of pesticide multi - residues.

关 键 词：固相萃取CARB/NH2柱 双柱-NPD气相色谱法 有机磷和氨基甲酸酯农药多残留 快速检测技术 

分 类 号：O657.71[理学—分析化学]