初筛抗-HIV1+2阳性而确认阴性标本的分析  

Analysis of samples positive by routine HIV1+2 antibody screening test and negative by WB assay

作　　者：郑艳丽[1] 夏银祥[1] 张晓琦[1] 

机构地区：[1]河南省平顶山市疾病预防控制中心,河南平顶山467000

出　　处：《中国卫生检验杂志》2007年第11期2020-2021,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：了解平顶山市2004年1月～2007年5月艾滋病病毒（HIV）抗体检测中筛查试验假阳性结果的真实情况,更加合理地开展HIV抗体日常检测。方法：检测结果及判断按《全国艾滋病检测技术规范》进行,对比分析HIV抗体筛查试验为阳性而免疫印迹试验（WB）为阴性的标本的结果以及相关资料。结果：46份筛查试验为阳性反应、WB确认试验为HIV抗体阴性的标本,占所有检测标本的1.45%,占筛查试验阳性标本的7.57%。46份标本中,胶体硒诊断试剂（PA）阳性的9份,占19.57%;酶联免疫吸附试验（ELISA）阳性40份,占86.96%,S/CO值平均为2.09（1.253～5.056）。结论：日常检测必须遵从先筛查再确认的检测程序,筛查试验阳性的不能直接出HIV抗体阳性报告,应确认后再报出。Objective ：To understand actual false positive results of HIV antibody screening test in Pingdingshan form 2004 to 2007 to improve current routine HIV screening practice and seek alternative approaches. Methods：In accordance with the Natinoal Technical Guideline for HIV/AIDS Detection, samples which were positive by routine HIV1 ＋ 2 antibody screening test and turned out negative by WB assay were analyzed and related data were reassessed. Results ：Fourty - six samples were positive by HIV antibody screening test but turned out negative by WB, accounting for 1.45% of all the tested samples and 7. 57% of all the positive ones. Of these 46 samples,9 were positive by PA test, accounting for 19. 57%. Of the 46 samples,40 were confirmed positiveby ELISA, accounting for 86. 96% . The lowest S/CO value was 1. 253 and the highest 5.056, with an average value of 2.09. Conclusion ：The routine HIV testing should follow the procedure that all screening tests be reconfirmed and that any HIV positive report cannot not be based on screening test finding alone.

关 键 词：艾滋病病毒抗体 免疫印迹试验 筛查试验 阳性反应 

分 类 号：R512.91[医药卫生—内科学]