利多卡因预先给药对内毒素性急性肺损伤大鼠肺组织层粘连蛋白表达的影响  

Effects of lidocaine pretreatment on expression of lung laminin in rats with LPS-induced acute lung injury

作　　者：张妍[1] 裴凌[2] 孙超[1] 

机构地区：[1]辽宁省抚顺矿业集团总医院麻醉科,113008 [2]中国医科大学附属第一医院麻醉科,沈阳市110001

出　　处：《中华麻醉学杂志》2007年第11期1019-1021,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30471666）;辽宁省教育厅基金资助项目（202013168）

摘　　要：目的探讨利多卡因预先给药对内毒素性急性肺损伤大鼠肺组织层粘连蛋白（LN）表达的影响。方法健康雄性Wistar大鼠32只,体重220～250g,随机分为4组（n=8）：对照组（C组）、脂多糖组（LPS组）、利多卡因2mg/kg组（L1组）和利多卡因4mg/kg组（L2组）。腹腔注射3%戊巴比妥钠20mg/kg麻醉后,建立尾静脉通路。C组尾静脉输注生理盐水3ml;LPS组依次尾静脉输注2ml生理盐水及1ml LPS（LPS 2mg/kg溶于1ml生理盐水）;利多卡因2、4mg/kg各溶于2ml生理盐水中,L_1组、L_2组分别先输注利多卡因再输注LPS,各组输注速率均为0.05ml/min。输注完毕后5h处死大鼠,迅速取右肺下叶肺组织,测定LN表达;取左肺上叶肺组织,透射电镜观察超微结构。结果与C组比较,LPS组肺组织LN表达上调（P〈0.05）;与LPS组比较,L1组和L2组肺组织LN表达下调（P〈0.05）;与L1组比较,L2组肺组织LN表达下调（P〈0.05）。L1组和L2组肺组织病理损伤较LPS组轻。结论利多卡因预先给药可通过抑制内毒素性急性肺损伤大鼠肺组织LN表达上调,减轻肺部炎性反应及肺损伤,且呈剂量依赖性。Objective To evaluate the effects of lidocaine pretreatment on the expression of lung laminin （LN） in rats with LPS-induced acute lung injury （ALI）.Methods Thirty-two male Wistar rats weighing 220-250 g were randomly divided into 4 groups （ n = 8 each） ： group Ⅰ control （C） ; group Ⅱ LPS; group Ⅲ lidocaine 2 mg/kg ＋ LPS （L1） and group Ⅳ lidocaine 4 mg/kg ＋ LPS （L2）. ALI was induced with LPS 2 mg/kg in 1 ml of normal saline infused iv over 20 min. In group Ⅲ and Ⅳ lidocaine 2 or 4 mg/kg was infused iv over 40 min followed by iv LPS. The animals were killed at 5 h after the end of LPS infusion. The lungs were removed for determination of LN expression by immuno-histochemical method and microscopic examination with electron microscope. Results Laminin was detected in all four groups. LPS induced significant increase in laminin expression in the lungs which was significantly attenuated by lidocaine pretreatment in group Ⅲ and Ⅳ in a dosedependent manner. The lung ultrastructure was badly damaged in group Ⅱ and lidocaine pretreatment greatly ameliorated the LPS-induced lung damage in group Ⅲ and Ⅳ . Conclusion Lidoeaine pretreatment can protect the lungs from LPS-induced injury by inhibiting excessive laminin expression in the lung.

关 键 词：利多卡因 内毒素血症 呼吸窘迫综合征 成人 层粘连蛋白 肺 

分 类 号：R686[医药卫生—骨科学]