甜菜M14品系特异表达基因ESTs的生物信息学分析  

Bioinformatical analysis of the ESTs of specially expressed genes in the apomictic M14 of sugar beet

作　　者：梅其文[1] 胡伟明[1] 吴烨东[1] 李海英[1] 

机构地区：[1]黑龙江大学生命科学学院分子生物学重点实验室,哈尔滨150080

出　　处：《黑龙江大学自然科学学报》2007年第5期682-686,688,共6页Journal of Natural Science of Heilongjiang University

基　　金：国家863计划项目(863-101-04-01-07);国家转基因科研计划项目(G99-A-014);黑龙江省自然科学基金重点项目(ZJN-03-6);黑龙江大学实验室开放项目

摘　　要：甜菜M14品系具有无融合生殖特性,为了获得M14品系特异表达的基因,本实验室前期利用抑制消减杂交(SSH)和mRNA差异显示(DDRT-PCR)技术对M14品系和能够进行有性生殖的栽培甜菜A2Y品系进行了基因特异表达研究,获得了298条M14品系特异表达基因的ES-Ts。对这些ESTs进行生物信息学分析并进行了GO分类,筛选出5条可能与基因表达调控和细胞周期调控相关的ESTs,为进一步获得这些基因的全长序列并研究其功能奠定了基础,也为今后能在甜菜M14品系中寻找并克隆与无融合生殖相关的基因提供了可靠的保证。M14 is a monosomic addition line with the characteristic of apomixis. By using the methods of suppression subtractive hybridization （ SSH ） and mRNA differential display reverse transcriptional PCR （ DDRT - PCR） between M14 and A2Y, 298 ESTs of specially expressed genes of M14 were obtained and the relevant bioinformatics analysis was made. Then, they were annotated and classified by GO （ Gene ontology） method, 5 ESTs whose function were closely related to regulation of gene expression and cell cycle. The research is the basis for obtaining full - length cDNA of these ESTs and characterizing their protein functions in the further study.

关 键 词：甜菜M14品系 EST(Expressed sequence tags) 生物信息学分析 GO(Gene Ontology) 

分 类 号：S566.3[农业科学—作物学] S188