明胶/PEG制备多孔块状SiO_2载体及葡萄糖淀粉酶的固定  

Synthesis and Glucoamylase Immobilization of SiO_2 Monolithic Carriers by Addition of Gelatin/PEG

作　　者：王彦芳[1] 陈奇[1] 叶蕊芳[2] 宋鹂[3] 张金朝[3] 候凤珍[3] 

机构地区：[1]华东理工大学超细材料制备与应用教育部重点实验室,上海200237 [2]华东理工大学生物反应器工程国家重点实验室,上海200237 [3]华东理工大学无机材料系,上海200237

出　　处：《无机化学学报》2007年第11期1959-1964,共6页Chinese Journal of Inorganic Chemistry

基　　金：上海市科委科技攻关项目(No.0252NM006)

摘　　要：本文通过溶胶-凝胶法,以正硅酸乙酯、聚乙二醇和明胶为主要原料,制得了孔径约为20nm的生物酶载体用多孔SiO2块状材料,并着重研究了热处理温度对多孔SiO2载体的结构和酶活力的影响。明胶的加入能有效地防止SiO2表面羟基在烧结过程中的失活现象,提高了多孔材料的吸附能力。制备过程中未加入明胶样品(G0)的酶吸附量和初始酶活力回收率的最高值分别为90mg·g-1和11%;加入质量分数为15%明胶的样品(G15)的酶吸附量和初始酶活力回收率分别提高到115mg·g-1和38%以上。经过5次重复使用后,G15载酶块体所显示的酶活力还高于G0载酶块体的初始酶活力。600℃热处理后所制得的G15样品表现出较好的综合性能,此类多孔块体材料便于酶的回收、保存和再利用。Mesoporous SiO2 monoliths with pore diameter of -20 nm for enzyme immobilization were synthesized via sol-gel route by using tetraethoxysilane （TEOS）, polyethylene glycol （PEG） and gelatin as raw materials. Effect of heating temperature on the structure and enzyme activity of porous SiO2 monoliths was studied. Gelatin was added to prevent hydroxyl groups on pore surface of SiO2 monolith from deactivation in heating process, and therefore to retain the strong absorption ability of SiO2 monoliths. The maximums of glucoamylase loading and activity retention were 90 mg·g^-1 and 11% for sample without adding gelatin （GO）, whereas the minimums of glucoamylase loading and activity retention of the sample with 15wt% gelatin content （G15） were 115 mg·g^-1 and 38%. The enzyme activities of G15 samples after reuse for 5 times was still higher than the primary enzyme activities of GO samples. Sample G15 heated at 600℃ exhibits better overall performance and such SiO2 porous monoliths for enzyme immobilization are easy to reclamation, reservation and recycle.

关 键 词：多孔SIO2 明胶 溶胶-凝胶 酶载体 

分 类 号：O613.72[理学—无机化学] TQ171[理学—化学]