阿特拉津高效降解菌的分离与筛选  

Isolation and Screening of Atrazine-degrading Bacteria from Soil

作　　者：应飞[1] 谢明[1] 万方浩[1] 王瑞[1] 邱卫亮[1] 李丽[1] 

机构地区：[1]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100094

出　　处：《中国生物防治》2007年第4期368-372,共5页Chinese Journal of Biological Control

基　　金：国家"十一.五"863计划(2006AA10Z401);科技支撑计划(2006BAD08A02;2006BAD08A08)

摘　　要：土样采自河北、山东一些农药厂排污口,通过室内阿特拉津无机盐培养基的驯化培养,分离得到4株在无机盐培养基上对阿特拉津有明显降解圈的降解细菌。底物阿特拉津浓度为1000mg/L,反应体系50ml,体系菌浓度为8.9×10^7cfu/ml,恒温30℃,180r/min,培养7d,其室内降解效率分别为40.6%、75.7%、82.3%、96.9%。其中菌株BZB-11的降解效率最高。对菌株BZB-11进行降解动态考察,结果显示,BZB-11菌株在无机盐液体培养基中（阿特拉津底物浓度为1000mg/L,反应体系50ml,体系菌浓度6.59×10^9cfu/ml,恒温30℃,180r/min）,对阿特拉津1-3d的降解速度较快,3d可达89.5%,7d的降解率达100%。综合来看,该菌株是一株很有应用前景的高效菌株。In laboratory four bacterial isolates degrading atrazine were isolated from the sludge around the chemical factories in Hebei and Shandong Provinces on atrazine mineral medium. They were differentiated by observing clear zones on indicator agar plate containing 1000 mg/L atrazine. Their capacity of degradation to atrazine was at the percentages of 40.6%, 75.7%, 82.3%, 96.9% respectively （8.9 × 10^7 cfu/ml, atrazine 1000 mg/L, mineral medium 50 ml, 180 r/min, 30℃, 7 d）. One of them, isolate BZB-11, showed the highest rate of degradation. Investigation of the trend of degradation showed the higher degrading rate （89.5%） within first 3 d, and reached a percentage of 100% of degradation within 7 d （6.59 × 10^9 cfu/ml, atrazine 1000 mg/L, mineral medium 50 ml）. In general, the isolate, BZB- 11, would be a potentially effective bacterium for herbicide contamination bioremediation.

关 键 词：阿特拉津 降解菌 分离筛选 生物降解 农药残留 

分 类 号：X592[环境科学与工程—环境工程] X172