犬细小病毒四川株的分离与鉴定  

Isolation and identification of canine parvovirus Sichuan strains

作　　者：戈锐[1] 彭广能[1] 夏咸柱[2] 杨松涛[2] 苏建青[2] 邹啸环[2] 冯娜[2] 马晓平[1] 石锦江[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]军事科学院兽医研究所,吉林长春130062

出　　处：《中国预防兽医学报》2007年第11期836-839,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：全军医药卫生科技攻关课题"军犬重要疫病联合重组活疫苗的研发"(06G138)

摘　　要：为更好地了解我国细小病毒流行情况,笔者从四川农大动物医院采集5份患出血性肠炎犬的粪便,通过猫肾细胞(F81)传代培养后,成功分离到两株犬细小病毒。对两株病毒进行病毒形态学、理化学、血凝试验、PCR鉴定与分子生物学系统鉴定后,证实分离株是犬细小病毒,通过VP2结构蛋白测序分析表明,新分离到的两株细小病毒抗原型分别属于CPV-2a和CPV-2b。To better understand the prevalence of CPV in China, we collected five stool samples from clinical dogs with haemorrhagic enteritis in animal hospital. Two virus strains were isolated by passaging in F81 cells, The viruses were identified as canine parvovirus based on morphology, serology and PCR results, According to sequence analysis of VP2 gene, the antigenic types of the new isolated CPVs belong to CPV-2a and CPV-2b respectively.

关 键 词：犬细小病毒 分离 鉴定 

分 类 号：S852.659.1[农业科学—基础兽医学]