猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定  

Preparation and characterization of the monoclonal antibodies against neutralizing epitope region of PEDV S protein

作　　者：孙东波[1] 冯力[1] 时洪艳[1] 陈建飞[1] 崔晓辰 佟有恩[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2007年第11期887-890,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：以纯化的猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)S蛋白中和表位(SID)重组蛋白为抗原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术获得了6株稳定分泌抗SID区特异性的单克隆抗体细胞株,分别命名为2C4、3G3、5F8、3G5、6E6和3C3。6株杂交瘤细胞诱生小鼠产生的腹水抗体效价分别为1∶51200、1∶6400、1∶12800、1∶6400、1∶51200和1∶25600。免疫球蛋白类型均为IgG1型,轻链均为Κ链。Western blot试验结果显示,6株单克隆抗体均能特异性地识别天然PEDV中的S蛋白。The purified neutralizing epitope region of PEDV S protein, S1D, was used to immunize BALB/c mice for cell fusion. Six hybridoma cell lines secreting monoclonal antibodies （McAbs） against S1D were established and designated as 2C4, 3G3, 5F8, 3G5, 6E6 and 3C3, respectively. All six McAbs were lgG1 subtype and had K chain. The McAbs had ascetic titers of 1：51 200, 1：6 400, 1：12 800, 1：6 400, 1：51 200 and 1：25 600 respectively. Western blot confirmed that all McAbs were able to reacted with native S protein of PEDV.

关 键 词：猪流行性腹泻病毒 S蛋白 中和表位区 单克隆抗体 

分 类 号：S852.659.6[农业科学—基础兽医学]