复合系WSC-6的菌种组成特性及其木质纤维素分解能力  

Microbial component diversity and capacity of lignocellulose degradation of composite bacterial system WSC-6

作　　者：王伟东[1] 王小芬[2] 李玉花[1] 刘长莉[1] 李文哲[3] 崔宗均[2] 

机构地区：[1]东北林业大学生命学院 [2]中国农业大学农学与生物技术学院,北京100094 [3]东北农业大学工程学院,哈尔滨150030

出　　处：《农业工程学报》2007年第10期210-215,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金项目(30571088);国家"十一五"科技支撑项目(2006BAD07A10;2006BAD04A09);黑龙江省博士后基金项目(LDH-Z05009);黑龙江省普通高等学校青年学术骨干支持计划项目(1151G030);黑龙江省"十一五"科技重点攻关项目(GB06B502);黑龙江省农垦总局科技攻关项目(HNKXIV-02-03-03)

摘　　要：利用平板分离法与变性梯度凝胶电泳(DGGE)法研究了高效分解木质纤维素的微生物复合系WSC-6的菌种组成,平板法分离得到了9株好气性的细菌,它们与Pseudoxanthomonas taiwanensis、Tepidiphilus margaritif er、Bacillus sp.E53-10、Proteobacterium S072、Beta proteobacterium HMD444、Rhizobiaceae str.M100、Petrobacter succinimandens BON4、Bacillus thermoamylovorans、Paenibacillus sp.SAFN-016的相似率分别达到了97.5%,99.0%,98.4%,100%,98.9%,99.3%,98.1%,99.5%,99.8%。利用DGGE分析表明,复合系中还存在利用平板法难以培养的5株厌氧或者兼性厌氧细菌,它们的16SrDNA V3区的序列与Ureibacillus thermosphaericu、Clostridium thermosuccinogenes、Clostridium thermopalmarium、Uncultured Clostridium sp.clone A1-3、Uncultured bacterium tbr4-24具有很高的相似率。在50℃静置培养条件下,接种3d后复合系WSC-6可以分解添加稻秆总量的81.3%。好氧菌与厌氧菌共存于复合系中,复合系表现出了高的细菌组成多样性,从而保证了复合系具有强的稳定性和多菌协同分解木质纤维素的能力,明确菌种组成多样性对于研究复合系高效分解木质纤维素的机理意义重大。Microbial component diversity of a composite bacterial system, being capable to degrading lignocellulose efficiently, was analyzed using denaturing gradient gel electrophoresis（DGGE） on the basis of 16SrDNA and plating. Nine aerobic bacterial isolates, isolate-1 to isolate-9, were isolated from peptone cellulose solution medium using plate cultivation, and the similarities of sequences of 16SrDNA between nine pure isolates with Pseudoxanthomonas taiwanensis, Tepidiphilus margaritifer, Bacillus sp. E53-10, Proteobacterium S072, Beta proteobacterium HMD444, Rhizobiaceae str. M100, Petrobacter succinimandens BON4, Bacillus thermoamylovorans, Paenibacillus sp. SAFN-016 were 97.5%, 99.0%, 98.4%, 100%, 98.9%, 99.3%, 98.1%, 99.5%, 99.8%, respectively. In addition, five bands of band A, band B, band C, band D and band E were detected using DGGE besides nine bands of pure isolates, and similarities of 16SrDNA V3 region betwen five bands with Ureibacillus thermosphaericu, Clostridium thermosuccinogenes, Clostridium thermopalmarium, Uncultured Clostridium sp. clone A1-3 and Uncultured bacterium tbr4-24 were 100%, 95.3%, 96.3%, 97.0% and 95.7%, respectively. After inoculating, 81.3% of rice straw weight was degraded under static condition within the first three days at 5started to get heat, at 13∶30 it was at the peak of getting heat 34.9 W/m2; from 18∶30 to the next morning, it lost heat. In the overcast day, the biggest and smallest values were reduced. In the cloudy day, the result was within the clear day and the overcast day, and it contained clear fluctuation. The heat convection was bigger than the heat conduction.

关 键 词：木质纤维素分解 微生物复合系 菌种组成多样性 变性梯度凝胶电泳 

分 类 号：X705[环境科学与工程—环境工程]