补骨脂雌激素样作用的有效成分研究  

Study on the Effective Constituents with Estrogenic Action in Fructus Psoraleae

作　　者：寿清耀[1] 杨荣平[2] 王宾豪[2] 刘剑毅[2] 徐嘉红[2] 

机构地区：[1]上海中医药大学,上海201203 [2]重庆市中药研究院,重庆400065

出　　处：《中药新药与临床药理》2007年第6期425-427,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：重庆市自然科学基金项目(CSTC;2005AD5020)

摘　　要：目的筛选中药补骨脂类雌激素样作用的有效成分。方法采用系统溶剂法对补骨脂药材进行分段制样,通过小鼠子宫增重法筛选出活性部位;对石油醚部位进行硅胶柱色谱分离,通过小鼠子宫增重法筛选出有效成分。结果补骨脂石油醚提取部位高剂量组(5g/kg)能显著增加子宫重量和子宫系数,与正常对照组相比有显著性差异(P<0.01),具有较强的类雌激素样作用;从补骨脂石油醚提取部位分离得到的化合物补骨脂酚具有显著的雌激素样活性(P<0.01)。结论补骨脂酚为中药补骨脂雌激素样作用的有效成分之一。Objective To screen out the effective constituents with estrogenic action in Fructus psoraleae. Methods Fructus psoraleae was separated by using systemic solvent distillation method, and the effective fraction was screened out by the experiment of increasing the uterine weight in mice. Then the petroleum fraction was separated by sil; ca gel chromatrography and the effective constituent was screened by the experiment of increasing the uterine weight in mice. Results High-dose（5g/kg） of the petroleum fraction showed obvious estrogenic action （P 〈 0. 01） compared with the blank group; Bakuchiol separated from the petroleum fraction showed markedly estrogenic action too（ P 〈 0.01）. Conclusion Bakuchiol is one of the effective constituents with estrogenic action in Fructus Psoraleae.

关 键 词：补骨脂 类雌激素样作用 有效成分 

分 类 号：R285.5[医药卫生—中药学]