Synthesis of N-methylene phosphonic chitosan(NMPCS)and its potential as gene carder  

Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier

作　　者：Dun Wan Zhu Jin Gen Bo Hai Ling Zhang Wen Guang Liu Xi Gang Leng Cun Xian Song Yu Ji Yin Li Ping Song Lan Xia Liu Lin Mei Xiu Lan Li Yang Zhang Kang De Yao 

机构地区：[1]Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China [2]Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Tianjin 300192, China [3]Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China

出　　处：《Chinese Chemical Letters》2007年第11期1407-1410,共4页中国化学快报（英文版）

基　　金：supports from National Natural Science Foundation of China(No.50233020,30300086);the financial support from Natural Science Foundation of Tianjin(No.05YFJMJC10200).

摘　　要：N-Methylene phosphonic chitosan （NMPCS）, an amphiphilic macromolecule with powerful chelating ability of Ca^2＋ ions, was synthesized and characterized. The physicochernical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR, ^13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy （AFM） and circular dichroism （CD）. The results suggest that at charge ratio 2：1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.N-Methylene phosphonic chitosan （NMPCS）, an amphiphilic macromolecule with powerful chelating ability of Ca^2＋ ions, was synthesized and characterized. The physicochernical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR, ^13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy （AFM） and circular dichroism （CD）. The results suggest that at charge ratio 2：1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80-210 nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.

关 键 词：Non-viral vector Gene delivery N-Methylene phosphonic chitosan 

分 类 号：O62[理学—有机化学]