光谱法研究吡嗪酰胺与牛血清白蛋白的相互作用  

Spectrophotometric study on the interaction between pyrazinamide and bovine serum albumin

作　　者：张霞[1] 倪永年[1] 

机构地区：[1]南昌大学化学系,江西南昌330047

出　　处：《化学研究与应用》2007年第11期1211-1214,共4页Chemical Research and Application

基　　金：国家自然科学基金(20562009)资助项目;江西省自然科学基金(0620041)资助项目;中科院电分析化学和湖南大学化学传感与计量学国家重点实验室基金(SKLEAC2004-3和CBC2005-22)资助项目;长江学者及创新团队项目(IRT0540)资助项目

摘　　要：用光谱法研究了抗结核药吡嗪酰胺(Pyrazinamide,PZA)与牛血清白蛋白(bovine serum albumin,BSA)的相互作用,初步探讨了PZA对BSA的猝灭机理为静态猝灭,并用荧光猝灭法测定了作用的位点数n和结合常数K。根据不同温度下的热力学常数确定了PZA与BSA之间的作用力主要是氢键和范德华力作用。根据Forster非辐射能量转移原理,测定了实验条件下PZA与BSA相互结合时,能量授体-受体的结合距离。并用同步荧光和紫外光谱法研究了PZA对BSA构象的影响。The binding of Pyrazinamide（PZA）, generally used as an antitubercular drug, to bovine serum albumin （BSA） has been investigated by spectrometry. The quenching mechanism of fluorescence of BSA by PZA was found to be static quenching, and the binding sites number n and apparent binding constant K was measured by fluorescence quenching method. Thermodynamic pa- rameters obtained from measured data at different temperatures showed that the binding of PZA to BSA involves hydrogen bond and Van der Waals＇ interation. The binding distance was obtained according to the theory of Forster＇s non - radiation energy transter. The effect of PZA on the conformation of BSA was also analyzed using synchronous fluorescence spectrometry and absorption spectrometry.

关 键 词：吡嗪酰胺 牛血清白蛋白 相互作用 

分 类 号：O657.3[理学—分析化学]