FSH对仔猪睾丸支持细胞增殖和GDNF表达的调节  

Regulation of FSH on proliferation of piglet testis sertoli cell and expression of GDNF

作　　者：孙燕[1] 王鲜忠[1] 吴建云[2] 白汝岚[2] 张家骅[1] 

机构地区：[1]西南大学牧草与草食家畜重点实验室,重庆400716 [2]西南大学动物科技学院,重庆400716

出　　处：《中国兽医科学》2007年第11期982-986,共5页Chinese Veterinary Science

基　　金：教育部博士学科点专项基金(20040625004);重庆市教委项目(KJ060314)

摘　　要：以原代培养的仔猪睾丸支持细胞(SC)为试验模型,研究了FSH对SC胶质细胞源性神经营养因子(GDNF)表达及其增殖的影响。结果显示,外源性添加FSH可促进GDNF蛋白表达,而且这种促进作用具有浓度和时间依赖关系,GDNF蛋白水平随着FSH浓度的增加而升高,当FSH浓度为50 ng/mL时,GDNF水平达到最高,然后逐渐降低;FSH(50 ng/mL)作用30 min,可显著促进GDNF蛋白的表达(P<0.05),当FSH作用1 h时,GDNF蛋白水平达到最高,随后逐渐降低。FSH可以时间-剂量依赖性促进SC增殖细胞核抗原(PCNA)的表达,FSH(50 ng/mL)作用30 min时,PCNA的表达显著增加(P<0.05),作用1 h达极显著水平(P<0.01),至2 h时,PCNA蛋白的表达水平达到最高,随后逐渐降低。提示,FSH可在体外条件下通过刺激SC表达GDNF进而促进其增殖。Primary piglet sertoli cells（SCs） were used to analyze the effects of FSH on the proliferative potential and GDNF expression of SCs. The FSH-stimulated GDNF expression in the cultured SCs was time- and concentration-dependent,that was the expression of GDNF protein reached its highest level when the concentration of FSH was 50 ng/mL. The protein expression was increased significantly when the stimulating time was 30 min and reached a peak level at hour 1, then declined gradually. FSH increased expression of proliferating cell nuclear antigen（PCNA） in SCs. Expression level of PCNA protein increased significantly at 30th rain and reached a peak at hour 2,then declined gradually. It was concluded that FSH was able to enhance proliferation of SCs in time- and dose-dependent manners by stimulating expression of GDNF.

关 键 词：促卵泡素 睾丸支持细胞 胶质细胞源性神经营养因子(GDNF) 增殖细胞核抗原 

分 类 号：S814.1[农业科学—畜牧学]