慢性复合应激对大鼠胃幽门HAP1表达的影响  

Effect of chronic multiple stress on the expression of HAP1 in the stomachus pyloricus of rats

作　　者：廖义林[1] 廖家万[1] 刘能保[2] 李晓恒[2] 

机构地区：[1]井冈山大学医学院组织胚胎学教研室,江西吉安343000 [2]华中科技大学同济医学院组织胚胎学教研室,湖北武汉430030

出　　处：《第四军医大学学报》2007年第22期2029-2030,共2页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30270446)

摘　　要：目的:探讨慢性复合应激大鼠胃幽门内分泌细胞亨廷顿蛋白相关蛋白1(HAP1)表达的变化及其意义.方法:大鼠12只随机分为慢性复合应激组和正常对照组.应激组动物进行6wk的垂直旋转、睡眠剥夺、捆绑(6h/d)和夜间光照等慢性复合性应激试验;实验结束后,所有动物采用免疫组织化学和Western Blot等方法检测胃幽门内分泌细胞内HAP1蛋白表达的变化.结果:HAP1在大鼠胃幽门主要表达在胃泌素细胞中.与对照组相比,慢性复合应激组大鼠胃幽门内分泌细胞内HAP1的表达增强(P<0.05).结论:慢性复合性应激大鼠HAP1在胃幽门胃泌素细胞的表达加强.AIM： To investigate the changes of the expression of HAP1 in the stomachus pyloricus of the rats exposed to chronic multiple stress and its significance. METHODS： Twelve adult rats were randomly divided into 2 groups： Chronic multiple stress group and control group. Rats in the multiple stress group were irregularly and alternatively exposed to the situation of vertical revolve, sleep deprivation, restraint （6 h/d）, and night illumination for 6 weeks to prepare chronic multiple stressed model. The expression of HAP1 protein in the stomachus pyloricus of rats was detected by immunohistochemistry. RESULTS： HAP1 was mainly distributed in the gastrin-secreting endocrine cells. Compared with control group, the immunoreactivities of HAP1 in the stomathus pyloricus increased in multiple stressed group （ P 〈 0.05 ）. CONCLUSION： Chronic multiple stress increases the expression of HAP1 protein in the stomachus pyloricus of the rat.

关 键 词：慢性复合应激 亨廷顿蛋白相关蛋白1 幽门 大鼠 

分 类 号：R338.6[医药卫生—人体生理学]