双歧杆菌对轮状病毒感染肠上皮细胞IL-8和TNF-α分泌的影响  

Effect of bifidobacteria on IL-8 and TNF-α secretion in rotavirus infected intestinal epithelial cells

作　　者：黄鸿眉[1] 程茜[1] 

机构地区：[1]重庆医科大学附属儿童医院儿童保健科,重庆400014

出　　处：《第四军医大学学报》2007年第22期2054-2056,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨双歧杆菌对轮状病毒(RV)体外感染肠上皮细胞株HT29过程中IL-8,TNF-α分泌水平的影响.方法:按照不同的干扰措施将细胞分为正常细胞对照、RV感染、低剂量双歧杆菌干预、高剂量双歧杆菌干预4组.光镜下观察细胞病变,ELISA检测感染后6,24h细胞培养上清中IL-8,TNF-α的表达水平.结果:HT29细胞感染RV后6h,细胞培养上清中IL-8分泌较正常细胞对照增加(P<0.01),而TNF-α分泌量无显著变化(P>0.05);感染后24h,两者分泌量皆显著增高(P<0.05),细胞发生显著病变,高、低剂量双歧杆菌干预细胞均较RV感染细胞病变程度轻.干预细胞与RV感染细胞比较,6h时IL-8,TNF-α分泌量都未出现明显变化(P>0.05),24h时,高、低剂量干预细胞的IL-8,TNF-α分泌水平皆较RV感染细胞明显降低(P<0.05),两种剂量组间无显著差别(P>0.05).结论:RV感染诱导肠上皮细胞株HT29大量分泌致炎性细胞因子IL-8,TNF-α,双歧杆菌能够显著抑制该刺激分泌作用,减轻细胞病变程度,提示这种抑制作用可能参与双歧杆菌抗RV感染的机制.AIM： To explore the possible mechanism of anti-rotavirus （RV） infection effect of bifidobacteria （Bb） through studying the effect of Bb on IL-8 and TNF-α secretion in RV infected epithelial cell line HT29. METHODS： Bb were anaerobically cultivated. HT29 cells were divided into 4 groups ： normal control group （NC group）, RV infection group （RI group）, RV infection ＋ low dose Bb group （ LB group） and RV infection ＋ high dose Bb group （HB group）. Inactivated Bb were respectively added into LB group and HB group with final concentration of 1 × 10^6 and 1 × 10^9 CFU/mL after HT29 cells were infected with RV. Cytopathic effect （CPE） was observed every day. Cell supematants were collected 6 and 24 h post RV infection, and examined for secretion levels of IL-8 and TNF-α by ELISA. RESULTS： There was no CPE in RI group, LB group, HB group 6 h post RV infection, but all the 3 groups showed CPE at 24 h and it was significantly lighter in the latter 2 groups than in the former one. Compared to NC group, the RI group had a higher IL-8 level （P 〈 0.01 ） and similar TNF-α level 6 h post infection （ P 〉 0.05 ）, but markedly higher levels of both two cytokines 24 h post infection （P 〈 0.05 ）. There was no obvious difference in IL-8 and TNF-α levels 6 h post infection between the RI group and Bb group （ P 〉 0.05 ）. The 2 Bb treatment groups, however, had significantly lower levels of both two proinflammatory cytokines than RI group 24 h post RV infection （ P 〈 0.05 ）. There was no remarkable difference in the 2 cytokines level between the high dose Bb group and the low dose Bb group 24 h post RV infection （ P 〉 0.05 ）. CONCLUSION： Proinflammatory cytokines of IL-8 and TNF-α in HT29 ceils may be involved in the damage of ceils. Bb can relieve the CPE and significantly decrease the proinflammatory cytokines level. It indicates that this inhibition effect probably be involved in the anti-RV infection mechanism of Bb.

关 键 词：二裂菌属 轮状病毒属 肠 上皮细胞 白细胞介素8 肿瘤坏死因子α 

分 类 号：R72[医药卫生—儿科]