高胰岛素-正常葡萄糖钳夹技术的建立  

Establishment of hyperinsulinemic-euglycemic clamp technique

作　　者：程时武[1] 陆菊明[1] 杨国庆[1] 李一君[1] 潘长玉[1] 翟淮伟 

机构地区：[1]解放军总医院内分泌科 [2]解放军62301部队门诊部

出　　处：《第四军医大学学报》2007年第22期2082-2084,共3页Journal of the Fourth Military Medical University

摘　　要：目的:建立能精确测定胰岛素敏感性的高胰岛素-正常葡萄糖钳夹技术.方法:应用高胰岛素-正常葡萄糖钳夹技术对10名正常体质量、正常糖耐量的健康志愿者进行方法学研究.结果:钳夹试验开始后,血浆胰岛素浓度迅速上升并维持在100mU/L左右,空腹血糖维持在正常水平(5.0mmol/L),试验过程中内源性胰岛素和肝糖源输出得到完全抑制,血液升糖激素浓度(包括皮质醇和生长激素)与基础值相比无明显上升.钳夹稳态期,葡萄糖输注率为(11.56±1.74)mg/(kg.min).结论:高胰岛素-正常葡萄糖钳夹技术已成功建立.在高胰岛素-正常葡萄糖稳态条件下,葡萄糖输注率等于机体的葡萄糖利用率,葡萄糖输注率可以作为评价组织对外源性胰岛素敏感性的指标.AIM ： To establish a hyperinsulinemic-euglycemic clamp technique, a method for the precise measurement of insulin sensitivity. METHODS： A hyperinsulinemic-euglycemic clamp technique was applied to the study of methodology in 10 healthy volunteers with normal body weight and glucose tolerance. RESULTS： The serum insulin concentration was acutely raised and maintained at approximately 100 mU/L. During maintaining fasting euglycemia （5.0 mmol/L）, the secretions of endogenous insulin and hepatic glucose production were completely inhibited. In comparison with basal values, the secretion of counter-regulatory hormones （including cortisol and growth hormone ） was not significantly stimulated. Under these steady-state conditions of hyperinsulinemia-euglycemia, the glucose infusion rate was （ 11.56 ± 1.74） mg/（ kg · min）. CONCLUSION： The hyperinsulinemiceuglycemic clamp technique is successfully established. Under these steady-state conditions of euglycemia, the glucose infusion rate equals to glucose disposal rate by all the tissues in the body and is therefore a index for tissue sensitivity to exogenous insulin.

关 键 词：胰岛素 葡萄糖钳制技术 胰岛素抗药性 胰岛素敏感性 

分 类 号：R587.1[医药卫生—内分泌]