抗氧化剂与力竭运动对大鼠肾脏自由基代谢和细胞凋亡的实验研究  

EFFECT OF EXHAUSTIVE EXERCISE AND VITAMIN E SUPPLEMENTATION ON APOPTOSIS AND FREE RADICAL METABOLISM IN KIDNEYS OF RATS

作　　者：王秋海[1] 黄玉山[2] 邓树勋[2] 

机构地区：[1]嘉应学院体育系,广东梅州514015 [2]华南师范大学体育科学学院,广东广州510631

出　　处：《华南师范大学学报（自然科学版）》2007年第4期114-119,共6页Journal of South China Normal University(Natural Science Edition)

摘　　要：观察力竭运动与抗氧化剂(维生素E)对肾脏细胞凋亡和自由基代谢的影响,以进一步阐明肾脏细胞凋亡的机制.将18只SD大鼠随机分为3组:安静对照组、力竭运动组和补充维生素E且力竭运动组.力竭运动24 h后取材,利用原位末端标记法(TUNEL法)测定大鼠肾脏细胞凋亡发生率,同时测定大鼠肾脏组织丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化酶(GSH-Px).结果发现:急性力竭运动导致肾小管远端细胞凋亡,但一次性补充抗氧化剂对肾脏细胞凋亡与自由基代谢影响不大,而且自由基代谢与肾脏细胞凋亡关系不大.To explore the effect of exhaustive exercise and Vitamin E supplementation on renal apoptosis and oxidative free radical mechanism of rats, 18 SD rats were randomly divided into three groups： sedentary control group, exhaustive exercise group and exhaustive exercise group which supplies Vitamin E. Apoptosis of renal ceils was investigated by TUNEL and MDA, SOD and GSH - Px of kidneys were investigated too. It observed that apoptosis of renal tubular increased during exhaustive exercise. But Vitamin E supplementation only temporary is useless.

关 键 词：力竭运动 细胞凋亡 氧自由基 肾脏 

分 类 号：Q95-3[生物学—动物学]