水溶性桥联双卟啉与DNA相互作用的研究  

Studies on Interaction of Water-Soluble Bridged Porphyrin with DNA

作　　者：王凯[1] 张智[1] 郭茜妮[1] 鲍小平[1] 李早英[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《化学学报》2007年第22期2597-2603,共7页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20672082)资助项目.

摘　　要：以4,4'-二羧酸-2,2'-联吡啶为桥联试剂,合成了一种含8个阳离子的水溶性桥联双卟啉(PD).以5,10,15,20-四(4-N-甲基吡啶盐)卟啉(H2TMPyP)为参照物,使用紫外-可见光谱、荧光光谱、圆二色谱研究了水溶性双卟啉与小牛胸腺DNA(CTDNA)的相互作用,以溴化乙啶(EB)竞争法测定了PD与CT DNA的表观键合常数(Kapp)为1.2×106L·mol-1(H2TMPyP为6.9×106L·mol-1),并使用凝胶电泳研究了PD对pBR322质粒DNA的切割能力.实验结果表明PD与CTDNA的作用方式是插入和外部结合的混合模式.A water-soluble porphyrin dimer （PD） containing eight positive charges, bridged by 4,4′-dicarboxy-2,2′-bipyridyl, has been synthesized. Taking meso-tetrakis（N-methylpyridinium-4-yl）porphyrin （H2TMPyP） as a reference compound, interaction of the water-soluble porphyrin dimer with DNA was investigated by using absorption, fluorescence, CD spectroscopy, and the apparent affinity binding constant （gapp） of PD binding to CT DNA was measured to be 1.2×10^6 L·mol^-1by a competition method with ethidium bromide （EB） （that of H2TMPyP was 6.9×10^6 L·mol^-1）. The cleavage ability of PD to pBR322 plasmid DNA was studied by gel electrophoresis. The results suggest that the binding modes of PD and CT DNA involve in both of intercalation and outside binding.

关 键 词：双卟啉 DNA 表观键合常数 光切割 

分 类 号：R96[医药卫生—药理学]