抗日本血吸虫未成熟虫卵单克隆抗体的制备和鉴定  

Production and Characterization of Monoclonal Antibody Against Immature Egg of Schistosoma Japonicun

作　　者：汪世平[1] 周汩波[1] 沈国励[2] 曾庆华 刘国章[3] 李汶[1] 曾宪芳[1] 

机构地区：[1]湖南医科大学,长沙410078 [2]湖南大学博士后流动站 [3]第一军医大学

出　　处：《中国人兽共患病杂志》1997年第4期36-39,共4页Chinese Journal of Zoonoses

基　　金：卫生部优秀青年科技人才基金!95-2-30;卫生部血吸虫病重点项目!94-Y-05

摘　　要：采用日本血吸虫来成熟卵抗原（SIEA）免疫BALB／c小鼠，制备抗SIEA单克隆抗体。获得一株强阳性、高特异的抗未成熟虫卵的单抗（2B2），免疫球蛋白为IgG1类。免疫印迹分析表明该单抗单一识到日本血吸虫未成熟虫卵抗原中的50kDa分子。免疫荧光染色表明，与雌虫子宫内虫卵呈现强阳性反应，而不与成虫抗原交叉。A cell live 2B2 secreting specific monoclonal antibody was obtained by fusion of mye1oma cells SP2/O and spleen cells from BALB/c mice immunized with soluble immature egg antigen (SIEA) of Schistosorpa japonicum.Immunoglobulin isotype of 2B2 was IgG. Further chaxacterization of McAb 2B2 showed that 50 kDa molecule.in SIEA was recognized by 2B2 with Western blot assay. This molecule was only expressed in immature eggs in the uterus of female adults as demonstrated by indirect immunofluorescence test, but not crossreacted with adultworms itself of Schistosoma japonicum.

关 键 词：日本血吸虫 未成熟虫卵 单克隆抗体 ELISA 

分 类 号：R383.24[医药卫生—医学寄生虫学] R392－33[医药卫生—基础医学]