遗传型与生理型雄性不育小麦花药和旗叶蛋白质的比较研究  

Comparison on Proteins in Anther and Flag Leaf of Sterile Wheat Induced by Physiology and Heredity

作　　者：王永军[1] 张改生[2] 孙苏阳[1] 李海军[1] 

机构地区：[1]淮安市农科院,江苏淮安223001 [2]西北农林科技大学,陕西杨凌712100

出　　处：《麦类作物学报》2007年第6期965-968,1022,共5页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(301705760);国家"863"重大专项(2002AA207004);陕西省"十五"攻关项目;国家杨凌农业生物技术育种中心专项基金项目

摘　　要：为了研究小麦遗传型和生理型雄性不育在生化机制上的异同,以遗传型[S型不育系ms(S)2611和ms(S)1376]和生理型雄性不育小麦西农2611和西农1376(高温诱导和化学杀雄剂SQ-1诱导的雄性不育)为材料,分别取其小孢子处于单核早期的幼穗,单核后期、二核期、三核期的花药及旗叶进行了可溶性蛋白质的比较研究。结果表明,从不同发育时期的旗叶可溶性蛋白中尚未发现特异蛋白的存在,但在花药中发现有特异蛋白的存在,表现在二核期生理型雄性不育及遗传型雄性不育材料均比正常的可育材料缺少了1条分子量为31 kD的多肽。这条多肽很可能是花粉发育过程中某一环节所必需的,缺少了它就形成不育,因此这条多肽很可能和育性相关。To study the similarities and differences between male-sterility induced by physiology and heredity,different male-sterile materials induced by heredity [S-type male-sterile line ms（S）2611,ms（S）1376]and physiology（induced by high temperature and chemical hybridizing agents SQ-1）,Xinong 1376 and Xinong 2611 were used as materials,and their spikes at mononucleated prophase stage,anther and flag leaf at mononucleated anaphase stage,binucleated stage and trinucleated stage were used to conduct protein analysis.There were on unique different protein observed in flag leaf at different development stage,but unique different protein were found in anther at binucleated stage and the male-sterile materials lack a 31 kD polypeptide compared with normal material.The 31 kD polypeptide perhaps is essential to the development of anther and is probably related to the male-sterilty.

关 键 词：小麦 雄性不育 化学杀雄 高温诱导 蛋白质 

分 类 号：S512.1[农业科学—作物学] S334.5