川产青牛胆的RAPD分析  

RAPD Analysis of Tinospora sagittata and T. sagittata var. craveniana Produced in Sichuan

作　　者：杨兵[1] 王晓静[1] 王天志[1] 罗禹[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《中国天然药物》2007年第6期428-430,共3页

摘　　要：目的:为川产青牛胆的物种鉴定和遗传图谱的构建寻找一种新的途径。方法:采用RAPD(Random Amplified Polymorphic DNA,随机扩增多态性DNA)技术对采自4个地区6个居群的32个DNA样品用筛选的15个引物进行扩增,进而进行聚类分析。结果:RAPD能将6个居群的样品明显区分开,居群内的遗传差异小于居群间差异,野生环境的样品遗传分化较大。结论:RAPD技术在分子水平上对青牛胆进行鉴定是一种行之有效的手段,从而为青牛胆物种鉴定、遗传图谱的构建等研究提供分子水平的方法和依据。AIM： To develop a new method for the species identification and genetic map construction of Tinospora sagittata （Oliv.） Gagnep＇s. METHODS：32 samples from 5 areas （6 clusters） have been amplified adopting 15 selected primers through applied clustering analysis： RESISTS： RAPD could differentiate samples from 6 different clusters. The inner cluster differences were smaller than exterior dusters. There were larger genetic differentiations among wild samples. CONCLUSION： RAPD is an effective method for T. sagittata （Oliv.） Gagnep＇s identification in molecular degree, and it has set up a foundation for the Tinospora sagittata （Oliv.） Gagnep＇s species identification and genetic map.

关 键 词：青牛胆 RAPD 分子标记 

分 类 号：R917[医药卫生—药物分析学]