蜜蜂囊状幼虫病毒病的Nest-PCR检测  

Nest-PCR Detection for the Honey bee Sacbrood Virus Disease

作　　者：许益鹏[1] 章奕卿[1] 李江红[1] 邢丽苹[1] 张传溪[1] 

机构地区：[1]浙江大学昆虫科学研究所,杭州310029

出　　处：《科技通报》2007年第6期824-827,共4页Bulletin of Science and Technology

基　　金：国家自然科学基金资助项目(30271008)

摘　　要：蜜蜂的囊状幼虫病是中华蜜蜂常见且危害严重的疾病,病原为一小正链RNA病毒。如何尽早鉴别蜂群的感染在养蜂业生产上具有重要的意义。本文报道一种利用RNA反转录结合巢式PCR检测鉴定蜜蜂的囊状幼虫病毒病的方法。Honey bee sacbrood virus （SBV） is a kind of picornavirus, a small positive strand RNA virus, which is greatly threatened to the Chinese honeybee breeding. How to detect the virus infection in early time is of great importance to bee breeding. We here report a sensitive method of integrating RNA reverse transcription and Nest-PCR to detect the virus infection. The results showed that the objected fragment could be successfully amplified from virus RNA genome prepared from the infected larvae.

关 键 词：中华蜜蜂 囊状幼虫病毒病 反转录 Nest—PCR 检测 

分 类 号：S432.1[农业科学—植物病理学]